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TNF-R2 (human):Fc (human), (recombinant)

 
ALX-522-014-C050 50 µg 414.00 USD
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Product Specification

Alternative Name:p75, CD120b, TNF-R 80kDa, TNFRSF 1B
 
MW:~75kDa (reducing SDS-PAGE).
 
Source:Produced in HEK 293 cells. The extracellular domain of human TNF-R2 (aa 1-257) is fused to the Fc portion of human IgG1.
 
UniProt ID:P20333
 
Concentration:1mg/ml after reconstitution.
 
Formulation:Lyophilized. Contains PBS.
 
Purity:≥95% (SDS-PAGE)
 
Endotoxin Content:<0.1EU/µg purified protein (LAL test; Bio Whittaker).
 
Species reactivity:Human, Mouse
 
Specificity:Binds human and mouse TNF-α (tumor necrosis factor-α).
 
Applications:ELISA
 
Application Notes:ELISA: binds to TNF-α.
Detection of membrane-bound TNF-α in combination with PAb to human IgG1 (e.g. ALX-211-502 (Biotin)).
 
Reconstitution:Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least 6 months after receipt when stored at -20°C.
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
 

Product Literature References

Development of improved soluble inhibitors of FasL and CD40L based on oligomerized receptors: N. Holler, et al.; J. Immunol. Methods 237, 159 (2000), Abstract;

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