Product Details
Alternative Name: | p75, CD120b, TNF-R 80kDa, TNFRSF 1B |
|
MW: | ~75kDa (reducing SDS-PAGE). |
|
Source: | Produced in HEK 293 cells. The extracellular domain of human TNF-R2 (aa 1-257) is fused to the Fc portion of human IgG1. |
|
UniProt ID: | P20333 |
|
Concentration: | 1mg/ml after reconstitution. |
|
Formulation: | Lyophilized. Contains PBS. |
|
Purity: | ≥95% (SDS-PAGE) |
|
Endotoxin Content: | <0.1EU/µg purified protein (LAL test; Bio Whittaker). |
|
Species reactivity: | Human, Mouse
|
|
Specificity: | Binds human and mouse TNF-α (tumor necrosis factor-α). |
|
Applications: | ELISA
|
|
Application Notes: | ELISA: binds to TNF-α.
Detection of membrane-bound TNF-α in combination with PAb to human IgG1. |
|
Reconstitution: | Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein. |
|
Shipping: | Blue Ice |
|
Long Term Storage: | -20°C |
|
Use/Stability: | Stable for at least 6 months after receipt when stored at -20°C. |
|
Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
|
Regulatory Status: | RUO - Research Use Only |
|
Product Literature References
Pigment epithelium-derived factor (PEDF) promotes tumor cell death by inducing macrophage membrane tumor necrosis factor-related apoptosis-inducing ligand (TRAIL): T.C. Ho, et al.; J. Biol. Chem.
286, 35943 (2011),
Abstract;
Full Text
Development of improved soluble inhibitors of FasL and CD40L based on oligomerized receptors: N. Holler, et al.; J. Immunol. Methods
237, 159 (2000),
Abstract;
Related Products