Product Details
| Alternative Name: | p55, CD120a, TNF-R 60kDa, TNFRSF 1A |
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| MW: | ~60kDa (reducing SDS-PAGE). |
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| Source: | Produced in HEK 293 cells. The extracellular domain of human TNF-R1 (aa 1-211) is fused to the Fc portion of human IgG1. |
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| UniProt ID: | P19438 |
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| Concentration: | 1mg/ml after reconstitution. |
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| Formulation: | Lyophilized. Contains PBS. |
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| Purity: | ≥95% (SDS-PAGE) |
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| Endotoxin Content: | <0.1EU/µg purified protein (LAL test; Bio Whittaker). |
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| Species reactivity: | Human, Mouse
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| Specificity: | Binds human and mouse TNF-α (tumor necrosis factor-a). |
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| Applications: | ELISA
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| Application Notes: | ELISA: binds to TNF-α. |
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| Reconstitution: | Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or carrier protein. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least 6 months after receipt when stored at -20°C. |
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| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
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| Technical Info/Product Notes: | Previous data has shown that TNF-R1 receptor binds to TNF-R1 and triggers various biological effects (including cell death). rhTNF-R1:Fc fusion protein blocks TNF-α-mediated lysis with a inhibitory activity in a concentration range of 10-50ng/ml using Wehi cells. |
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| Regulatory Status: | RUO - Research Use Only |
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Figure: Inhibition of TNF-mediated lysis of WEHI 164 target cells (rhsTNF-a, Prod. No.
ALX-522-008). rhTNF-R1:Fc exerts its inhibitory activity in a concentration range of 10-50ng/ml."
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Product Literature References
Proteasome inhibition triggers the formation of TRAIL receptor 2 platforms for caspase-8 activation that accumulate in the cytosol: C.T. Hellwig, et al.; Cell Death Differ.
29, 147 (2022),
Abstract;
TNF-α and IFN-γ are potential inducers of Fas-mediated keratinocyte apoptosis through activation of inducible nitric oxide synthase in toxic epidermal necrolysis: I. Viard-Leveugle, et al.; J. Invest. Dermatol.
133, 489 (2013),
Application(s): Flow cytometry analysis of NO production in mammalian cells,
Abstract;
Proteasome inhibition can induce an autophagy-dependent apical activation of caspase-8: M.A. Laussmann, et al.; Cell Death Differ.
18, 1584 (2011),
Abstract;
Development of improved soluble inhibitors of FasL and CD40L based on oligomerized receptors: N. Holler, et al.; J. Immunol. Methods
237, 159 (2000),
Abstract;
The lymphotoxin-β receptor is necessary and sufficient for LIGHT-mediated apoptosis of tumor cells: I.A. Rooney, et al.; J. Biol. Chem.
275, 13047 (2000),
Abstract;
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