TNF-R1 (human):Fc (human), (recombinant)



ALX-522-013-C050	50 µg	350.00 USD

Product Specification

Alternative Name:	p55, CD120a, TNF-R 60kDa, TNFRSF 1A

Concentration:	1mg/ml after reconstitution.

Endotoxin Content:	<0.1EU/µg purified protein (LAL test; Bio Whittaker).

MW:	~60kDa (reducing SDS-PAGE).

Purity:	≥95% (SDS-PAGE)

Formulation:	Lyophilized. Contains PBS.

Biological Activity:	Binds to TNF-R1 and triggers various biological effects (including cell death). rhTNF-R1:Fc fusion protein blocks TNF-α-mediated lysis with a inhibitory activity in a concentration range of 10-50ng/ml.

Source/Host:	Produced in HEK 293 cells. The extracellular domain of human TNF-R1 (aa 1-211) is fused to the Fc portion of human IgG1.

Reconstitution:	Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or carrier protein.

Specificity:	Binds human and mouse TNF-α (tumor necrosis factor-a).

Long Term Storage:	-20°C

Use/Stability:	Stable for at least 6 months after receipt when stored at -20°C.

Handling:	After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Background / Technical Information:	UniProt ID P19438: TNF-R1 (human)

Figure: Inhibition of TNF-mediated lysis of WEHI 164 target cells (rhsTNF-a, Prod. No. ALX-522-008). rhTNF-R1:Fc exerts its inhibitory activity in a concentration range of 10-50ng/ml.

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Product Literature References

Development of improved soluble inhibitors of FasL and CD40L based on oligomerized receptors: N. Holler, et al.; J. Immunol. Methods 237, 159 (2000), Abstract;

The lymphotoxin-β receptor is necessary and sufficient for LIGHT-mediated apoptosis of tumor cells: I.A. Rooney, et al.; J. Biol. Chem. 275, 13047 (2000), Abstract; Full Text