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TRAIL-R4 (human):Fc (human), (recombinant)

 
ALX-522-011-C050 50 µg 351.00 USD
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Product Specification

Alternative Name:DcR2, TNFRSF 10D, TRUNDD, CD264
 
MW:~54kDa (SDS-PAGE).
 
Source:Produced in HEK 293 cells. The extracellular domain of human TRAIL-R4 (DcR2) (aa 56-212) is fused to the Fc portion of human IgG1.
 
UniProt ID:Q9UBN6
 
Concentration:1mg/ml after reconstitution.
 
Formulation:Lyophilized. Contains PBS.
 
Purity:≥95% (SDS-PAGE)
 
Endotoxin Content:<0.1EU/µg purified protein (LAL test; Bio Whittaker).
 
Species reactivity:Human, Mouse
 
Specificity:Binds human and mouse TRAIL.
 
Biological Activity:Inhibits human TRAIL-mediated apoptosis.
 
Applications:ELISA
 
Application Notes:ELISA: binds to TRAIL at 10-100 ng/ml.
 
Reconstitution:Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least 6 months after receipt when stored at -20°C.
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
 
Technical Info/Product Notes:Historical data has shown inhibition of human TRAIL-mediated apoptosis in a concentration range of 2-10µg/ml. Use TRAIL-R2 (human):Fc (human) (rec.) (Prod. No. ALX-522-005) for the detection of surface human TRAIL by FC.
 
ALX-522-011a
The extracellular domain of human TRAIL-R4 is fused to the Fc portion of human IgG1.
alx-522-011
Figure: Inhibition of soluble human TRAIL (Prod. No. ALX-850-018)-mediated lysis. TRAIL-R4 (human):Fc (human) (rec.) fusion protein exerts its inhibitory activity in aconcentration range of 2-10µg/ml.Method: Human Jurkat lymphoma cells (50’000 cells in 100µl DMEM medium containing 5% fetal calf serum) were incubated with 0.1 µg/ml soluble TRAIL plus 2µg/ml enhancer and increasing concentrations of TRAIL-R4 (human):Fc (human) (rec.) in a 96-well plate for 16 hours at 37°C. Concentrations of TRAIL-R4 (human):Fc (human) (rec.) required to inhibit may vary depending on the cell type studied and on the concentration of sTRAIL used to kill cells. Cell viability was determined using a MTT-based cell proliferation assay kit.
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ALX-522-011a alx-522-011

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