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TNF-α (soluble) (mouse), (recombinant)

 
ALX-522-009-C050 50 µg 324.00 USD
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Product Specification

Alternative Name:Tumor necrosis factor-α, TNFSF 2
 
MW:~20kDa (SDS-PAGE).
 
Source:Produced in E. coli. The extracellular domain of mouse TNF-α (tumor necrosis factor-α) (aa 77-235) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG®-tag.
 
UniProt ID:P06804
 
Gene/Protein Identifier:A002291 (UCSD Signaling Gateway ID)
 
Concentration:1mg/ml after reconstitution.
 
Formulation:Lyophilized. Contains PBS.
 
Purity:≥90% (SDS-PAGE)
 
Endotoxin Content:<0.1EU/µg purified protein (LAL test; Associates of Cape Cod).
 
Species reactivity:Human, Mouse, Rat
 
Specificity:Binds to human, mouse and rat TNF-R1 and less efficiently to TNF-R2. In the presence of cross-linking enhancer (see Set Prod. No. ALX-850-061), TNF-α shows a significantly higher affinity for TNF-R2 than for TNF-R1, mimicking the characteristics of membrane-bound TNF-α.
 
Applications:ELISA
 
Application Notes:ELISA: binds to human TNF-R1 receptor.
 
Reconstitution:Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least 6 months after receipt when stored at -20°C.
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
 
Technical Info/Product Notes:Historical lots have shown that it exerts its biological activity in a concentration range of 0.5-1ng/ml (WEHI 164 cells). 

FLAG is a registered trademark of Sigma-Aldrich Co.
 
ALX-522-009 SDS-PAGE
SDS-PAGE Analysis of TNF-α (soluble) (mouse), (recombinant). Lane 1: MW Marker, Lane 2: 1 µg TNF-α
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ALX-522-009 SDS-PAGE

Product Literature References

A novel TNFR1-triggered apoptosis pathway mediated by class IA PI3Ks in neutrophils: B. Geering, et al.; Blood 117, 5953 (2011), Application(s): Death assay on mouse neutrophils, Abstract; Full Text
Inhibition of interleukin 1 receptor/Toll-like receptor signaling through the alternatively spliced, short form of MyD88 is due to its failure to recruit IRAK-4: K. Burns, et al.; J. Exp. Med. 197, 263 (2003), Abstract; Full Text
Conversion of membrane-bound Fas(CD95) ligand to its soluble form is associated with downregulation of its proapoptotic activity and loss of liver toxicity: P. Schneider, et al.; J. Exp. Med. 187, 1205 (1998), Abstract; Full Text

General Literature References

The transmembrane form of tumor necrosis factor is the prime activating ligand of the 80 kDa tumor necrosis factor receptor: M. Grell, et al.; Cell 83, 793 (1995), Abstract;
The TNF receptor superfamily of cellular and viral proteins: activation, costimulation, and death: C.A. Smith, et al.; Cell 76, 959 (1994), Abstract;
Tumor necrosis factor: a pleiotropic cytokine and therapeutic target: K.J. Tracey & A. Cerami; Annu. Rev. Med. 45, 491 (1994), Abstract;

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