TNF-α (soluble) (mouse), (recombinant)



ALX-522-009-C050	50 µg	283.00 USD

Product Specification

Alternative Name:	TNFSF 2, Tumor necrosis factor α

Concentration:	1mg/ml after reconstitution.

Endotoxin Content:	<0.1EU/µg purified protein (LAL test; Bio Whittaker).

MW:	~20kDa (SDS-PAGE).

Purity:	≥90% (SDS-PAGE)

Formulation:	Lyophilized. Contains PBS.

Biological Activity:	Exerts its biological activity in a concentration range of 0.5-1ng/ml (WEHI 164 cells).

Source/Host:	Produced in E. coli. The extracellular domain of mouse TNF-α (tumor necrosis factor-α) (aa 77-235) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG^®-tag.

Reconstitution:	Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.

Specificity:	Binds to human, mouse and rat TNF-R1 and less efficiently to TNF-R2. In the presence of cross-linking enhancer (see Set Prod. No. ALX-850-061), TNF-α shows a significantly higher affinity for TNF-R2 than for TNF-R1, mimicking the characteristics of membrane-bound TNF-α.

Long Term Storage:	-20°C

Use/Stability:	Stable for at least 6 months after receipt when stored at -20°C.

Handling:	After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Background / Technical Information:	UniProt ID P06804: Tumor necrosis factor (mouse) AfCS Signaling Gateway ID A002291: TNF-α (mouse) FLAG is a registered trademark of Sigma-Aldrich Co.

SDS-PAGE Analysis of TNF-α (soluble) (mouse), (recombinant). Lane 1: MW Marker, Lane 2: 1 µg TNF-α

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Product Literature References

Inhibition of interleukin 1 receptor/Toll-like receptor signaling through the alternatively spliced, short form of MyD88 is due to its failure to recruit IRAK-4: K. Burns, et al.; J. Exp. Med. 197, 263 (2003), Abstract; Full Text

Conversion of membrane-bound Fas(CD95) ligand to its soluble form is associated with downregulation of its proapoptotic activity and loss of liver toxicity: P. Schneider, et al.; J. Exp. Med. 187, 1205 (1998), Abstract; Full Text

General Literature References

The transmembrane form of tumor necrosis factor is the prime activating ligand of the 80 kDa tumor necrosis factor receptor: M. Grell, et al.; Cell 83, 793 (1995), Abstract;

The TNF receptor superfamily of cellular and viral proteins: activation, costimulation, and death: C.A. Smith, et al.; Cell 76, 959 (1994), Abstract;

Tumor necrosis factor: a pleiotropic cytokine and therapeutic target: K.J. Tracey & A. Cerami; Annu. Rev. Med. 45, 491 (1994), Abstract;