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TNF-α (soluble) (human), (recombinant)

ALX-522-008-C050 50 µg 338.00 USD
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Product Specification

Alternative Name:Tumor necrosis factor-α, TNFSF 2
MW:~19kDa (SDS-PAGE).
Source:Produced in E. coli. The extracellular domain of human TNF-α (tumor necrosis factor-α) (aa 85-223) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG®-tag.
UniProt ID:P01375
Concentration:1mg/ml after reconstitution.
Formulation:Lyophilized. Contains PBS.
Purity:≥95% (SDS-PAGE)
Endotoxin Content:<0.1EU/µg purified protein (LAL test; Associates of Cape Cod).
Species reactivity:Human, Mouse
Specificity:Binds to human and mouse TNF-R1 and less efficiently to TNF-R2. In the presence of cross-linking enhancer (Prod. No. ALX-804-034), TNF-α shows a significantly higher affinity for TNF-R2 than for TNF-R1, mimicking the characteristics of membrane-bound TNF-α.
Application Notes:ELISA: binds to TNF-R2 at 0.1-10 ng.
Reconstitution:Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Use/Stability:Stable for at least 6 months after receipt when stored at -20°C.
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Technical Info/Product Notes:Historical lots have demonstrated biological activity in a concentration range of 0.1-1ng/ml (WEHI 164 cells), and an ED50 of 0.1ng/ml (WEHI 164 cells).

FLAG is a registered trademark of Sigma-Aldrich Co.

Product Literature References

Metformin inhibits pro-inflammatory responses via targeting nuclear factor-κB in HaCaT cells: W. Ba, et al.; Cell Biochem. Funct. 37, 4 (2019), Abstract;
RIPK1 and Caspase-8 Ensure Chromosome Stability Independently of Their Role in Cell Death and Inflammation: G. Liccardi, et al.; Mol. Cell 73, 413 (2019), Abstract; Full Text
Proteasome inhibition blocks necroptosis by attenuating death complex aggregation: M. Ali, et al.; Cell Death Dis. 9, 346 (2018), Abstract; Full Text
MK2 Phosphorylates RIPK1 to Prevent TNF-Induced Cell Death: I. Jaco, et al.; Mol. Cell 66, 698 (2017), Abstract; Full Text
Cellular IAP proteins and LUBAC differentially regulate necrosome-associated RIP1 ubiquitination: M.C. de Almagro, et al.; Cell Death Dis. 6, e1800 (2015), Application(s): Cell Culture, Abstract; Full Text
Deubiquitinase-based analysis of ubiquitin chain architecture using Ubiquitin Chain Restriction (UbiCRest): M.K. Hospenthal, et al.; Nat. Protoc. 10, 349 (2015), Abstract; Full Text
Small-molecule modulators of the OX40-OX40L costimulatory protein-protein interaction: Y. Song, et al.; Br. J. Pharmacol. 171, 4955 (2014), Abstract;
De-ubiquitinating proteases USP2a and USP2c cause apoptosis by stabilising RIP1: A.L. Mahul-Mellier, et al.; Biochim. Biophys. Acta 1823, 1353 (2012), Abstract;
NF-kappaB regulates thrombin-induced ICAM-1 gene expression in cooperation with NFAT by binding to the intronic NF-kappaB site in the ICAM-1 gene: J. Xue, et al.; Physiol. Genomics 38, 42 (2009), Abstract; Full Text
The chemokine receptor CCR7 activates in dendritic cells two signaling modules that independently regulate chemotaxis and migratory speed: L. Riol-Blanco, et al.; J. Immunol. 174, 4070 (2005), Abstract; Full Text
BAFF production by antigen-presenting cells provides T cell co-stimulation: B. Huard, et al.; Int. Immunol. 16, 467 (2004), Abstract;
A second step of chemotaxis after transendothelial migration: keratinocytes undergoing apoptosis release IFN-gamma-inducible protein 10, monokine induced by IFN-gamma, and IFN-gamma-inducible alpha-chemoattractant for T cell chemotaxis toward epidermis in: S. Klunker, et al.; J. Immunol. 171, 1078 (2003), Abstract; Full Text
Induction of TNF receptor I-mediated apoptosis via two sequential signaling complexes: O. Micheau & J. Tschopp; Cell 114, 181 (2003), Abstract;
Insulin-secreting beta-cell dysfunction induced by human lipoproteins: M.E. Roehrich, et al.; J. Biol. Chem. 278, 18368 (2003), Abstract; Full Text
Conversion of membrane-bound Fas(CD95) ligand to its soluble form is associated with downregulation of its proapoptotic activity and loss of liver toxicity: P. Schneider, et al.; J. Exp. Med. 187, 1205 (1998), Abstract; Full Text

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ALX-804-034-C050 50 µg 273.00 USD
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