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TRAIL (soluble) (human), (recombinant)

 
ALX-522-003-C010 10 µg 228.00 USD
 
ALX-522-003-3010 3x10 µg SuperPack 467.00 USD
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Product Specification

Alternative Name:Apo-2L, TNFSF 10, CD253
 
MW:~28kDa
 
Source:Produced in E. coli. The extracellular domain of human TRAIL (aa 95-281) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG®-tag.
 
UniProt ID:P50591
 
Concentration:0.1mg/ml after reconstitution.
 
Formulation:Lyophilized. Contains PBS.
 
Purity:≥90% (SDS-PAGE)
 
Endotoxin Content:<0.1EU/µg purified protein (LAL test; Associates of Cape Cod).
 
Species reactivity:Human, Mouse
 
Specificity:Binds to human and mouse TRAIL receptors and human osteoprotegerin (OPG).
 
Biological Activity:Induces apoptosis if applied with the cross-linking enhancer (Prod. No. ALX-804-034); see also TRAIL, Soluble (human) (recombinant) Set (Prod. No. ALX-850-018). Note: rhsTRAIL does not significantly induce apoptosis in the absence of an enhancer.
 
Applications:ELISA
 
Application Notes:ELISA: binds to human TRAIL receptors and human osteoprotegerin (OPG) at 10-100 ng/ml.
 
Reconstitution:Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least 6 months after receipt when stored at -20°C.
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
 
Technical Info/Product Notes:Historical data has shown that Fas induces apoptosis in a concentration range of 1-100ng/ml if applied with the cross-linking enhancer (Prod. No. ALX-804-034).
 
522-003 SDS-PAGE
SDS PAGE Analysis: Lane 1: MW Marker, Lane 2: 1μg TRAIL.
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522-003 SDS-PAGE

Product Literature References

Inhibition of Notch1 signaling overcomes resistance to the death ligand Trail by specificity protein 1-dependent upregulation of death receptor 5: A. Fassl, et al.; Cell Death Dis. 6, e1921 (2015), Abstract; Full Text
Death receptor 5 signaling promotes hepatocyte lipoapoptosis: S.C. Cazanave, et al.; J. Biol. Chem. 286, 39336 (2011), Abstract;
A role for cFLIP in B cell proliferation and stress MAPK regulation: H. Zhang, et al.; J. Immunol. 182, 207 (2009), Abstract; Full Text
Cytoskeleton-mediated death receptor and ligand concentration in lipid rafts forms apoptosis-promoting clusters in cancer chemotherapy: C. Gajate & F. Mollinedo; J. Biol. Chem. 280, 11641 (2005), Abstract; Full Text
NF-κB signals induce the expression of c-FLIP: O. Micheau, et al.; Mol. Cell. Biol. 21, 5299 (2001), Abstract; Full Text
The cytokines tumor necrosis factor α (TNF-α) and TNF-related apoptosis-inducing ligand differentially modulate proliferation and apoptotic pathways in human keratinocytes expressing the h: J.R. Basile, et al.; J. Biol. Chem. 276, 22522 (2001), Abstract; Full Text
Tumor necrosis factor (TNF) and phorbol ester induce TNF-related apoptosis-inducing ligand (TRAIL) under critical involvement of NF-kappa B essential modulator (NEMO)/IKKgamma: D. Siegmund, et al.; J. Biol. Chem. 276, 43708 (2001), Abstract; Full Text
Combined effect of tumor necrosis factor-related apoptosis-inducing ligand and ionizing radiation in breast cancer therapy: A.M. Chinnaiyan, et al.; PNAS 97, 1754 (2000), Abstract;
FADD/MORT1 and caspase-8 are recruited to TRAIL receptors 1 and 2 and are essential for apoptosis mediated by TRAIL receptor 2: M.R. Sprick, et al.; Immunity 12, 599 (2000), Abstract;
Fas triggers an alternative, caspase-8-independent cell death pathway using the kinase RIP as effector molecule: N. Holler, et al.; Nat. Immunol. 1, 489 (2000), Abstract;
Conversion of membrane-bound Fas(CD95) ligand to its soluble form is associated with downregulation of its proapoptotic activity and loss of liver toxicity: P. Schneider, et al.; J. Exp. Med. 187, 1205 (1998), Abstract;
Characterization of two receptors for TRAIL: P. Schneider, et al.; FEBS Lett. 416, 329 (1997), Abstract;
Inhibition of death receptor signals by cellular FLIP: M. Irmler, et al.; Nature 388, 190 (1997), Abstract;
Viral FLICE-inhibitory proteins (FLIPs) prevent apoptosis induced by death receptors: M. Thome, et al.; Nature 386, 517 (1997), Abstract;

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Enhancer for Ligands 

Enhances biological activity of TRAIL, soluble (human) (recombinant) and FasL, soluble (human) (recombinant).
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ALX-804-034-C050 50 µg 260.00 USD
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