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Fas (human):Fc (human), (recombinant)

 
ALX-522-002-C050 50 µg 370.00 USD
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Product Specification

Alternative Name:CD95, APO-1, TNFRSF 6
 
MW:~56 kDa
 
Source:Produced in HEK 293 cells. The extracellular domain of human Fas (CD95; APO-1) (aa 7-154) is fused to the Fc portion of human IgG1.
 
UniProt ID:P25445
 
Concentration:1mg/ml after reconstitution.
 
Formulation:Lyophilized. Contains PBS.
 
Purity:≥95% (SDS-PAGE)
 
Purity Detail:Purified by multi-step chromatography.
 
Endotoxin Content:<0.1EU/µg purified protein (LAL test; Associates of Cape Cod).
 
Species reactivity:Human, Mouse, Rat
 
Specificity:Binds human, mouse and rat FasL (APO-1L; CD95L; CD178).
 
Applications:ELISA
 
Application Notes:ELISA: binds to FasL at 10-100 ng/ml.
 
Reconstitution:Reconstitute with 50µl sterile water. Further dilutions can be made with medium containing 5% fetal calf serum or a carrier protein.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least 6 months after receipt when stored at -20°C.
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
 
Technical Info/Product Notes:Historical data has shown that Fas inhibits the activity of human and mouse soluble Fas (sFasL) and thereby sFasL-mediated lysis of Fas sensitive cells in a concentration range of 20-100µg/ml. When used with cross-linking enhancer (see Prod. No. ALX-203-001), the inhibitory activity of rhFas:Fc is increased approximately 20- to 50-fold. Concentrations of rhFas:Fc required to inhibit may vary depending on the cell viability and on the concentrations of sFasL used to kill the cells.
 
850-016 522-001 002n
Figure: Inhibition of FasL, Soluble (human) (rec.) (Prod. No. ALX-522-001)-mediated lysis. Fas (human):Fc (human) (rec.) (Prod. No. ALX-522-002) exerts its inhibitory activity in a concentration range of 0.5-5µg/ml in the presence of the enhancer (1µg/ml).Method: Mouse A20 B lymphoma cells (50’000 cells in 100µl DMEM medium containing 5% fetal calf serum) were incubated with 0.2µg/ml FasL, Soluble (human) (rec.) and increasing concentrations of Fas (human):Fc (human) (rec.) in the presence and the absence of 1µg/ml enhancer) in a 96-well plate for 16 hours at 37°C. Concentrations of Fas (human):Fc (human) (rec.) required to inhibit may vary depending on the cell type studied and on the concentration of rhsFasL used to kill cells. Cell viability was determined using an MTT-based cell proliferation assay kit.
ALX-522-002 SDS-PAGE
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850-016 522-001 002n ALX-522-002 SDS-PAGE

Product Literature References

Proteasome inhibition can induce an autophagy-dependent apical activation of caspase-8: M.A. Laussmann, et al.; Cell Death Differ. 18, 1584 (2011), Abstract;
Th17 cells enhance viral persistence and inhibit T cell cytotoxicity in a model of chronic virus infection: W. Hou, et al.; J. Exp. Med. 206, 313 (2009), Abstract; Full Text
High expression of Fas ligand by synovial fluid-derived gamma delta T cells in Lyme arthritis: K. Roessner, et al.; J. Immunol. 170, 2702 (2003), Abstract; Full Text
Two adjacent trimeric Fas ligands are required for Fas signaling and formation of a death-inducing signaling complex: N. Holler, et al.; Mol. Cell. Biol. 23, 1428 (2003), Abstract; Full Text
Productive HIV-1 infection of primary CD4+ T cells induces mitochondrial membrane permeabilization leading to a caspase-independent cell death: F. Petit et al; J. Biol. Chem. 277, 1477 (2002), Abstract; Full Text
Mechanisms underlying neuronal death induced by chromogranin A-activated microglia: J. Ciesielski-Treska, et al.; J. Biol. Chem. 276, 13113 (2001), Abstract; Full Text
T cell costimulation by the TNF ligand BAFF: B. Huard et al.; J. Immunol. 167, 6225 (2001), Abstract;
Cysteine 230 is essential for the structure and activity of the cytotoxic ligand TRAIL: J.L. Bodmer, et al.; J. Biol. Chem. 275, 20632 (2000), Abstract; Full Text
Development of improved soluble inhibitors of FasL and CD40L based on oligomerized receptors: N. Holler, et al.; J. Immunol. Methods 237, 159 (2000), Abstract;
TGF-beta induces the expression of the FLICE-inhibitory protein and inhibits Fas-mediated apoptosis of microglia: R. Schlapbach, et al.; Eur. J. Immunol. 30, 3680 (2000), Abstract;
Characterization of Fas (Apo-1, CD95)-Fas ligand interaction: P. Schneider, et al.; J. Biol. Chem. 272, 18827 (1997), Abstract; Full Text

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