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FasL (soluble) (human), (recombinant)

Fas ligand
 
ALX-522-001-C010 10 µg 241.00 USD
 
ALX-522-001-3010 3x10 µg SuperPack 487.00 USD
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Product Specification

Alternative Name:APO-1L, CD95L, CD178, TNFSF 6
 
MW:~32kDa (non-glycosylated), ~35kDa (glycosylated) (SDS-PAGE).
 
Source:Produced in HEK 293 cells. The extracellular domain of human FasL (APO-1L; CD95L; CD178) (aa 103-281) is fused at the N-terminus to a linker peptide (26 aa) and a FLAG®-tag. Glycosylation of rhsFasL is similar to that of natural human FasL.
 
UniProt ID:P48023
 
Concentration:0.1mg/ml after reconstitution.
 
Formulation:Lyophilized. Contains PBS.
 
Purity:≥95% (SDS-PAGE)
 
Purity Detail:Purified by multi-step chromatography.
 
Endotoxin Content:<0.1EU/µg purified protein (LAL test; Associates of Cape Cod).
 
Species reactivity:Human, Mouse, Rat
 
Specificity:Binds to human, mouse and rat Fas (CD95; APO-1).
 
Biological Activity:Kills Jurkat cells in the absence of a cross-linker. Cross-linking enhancer (see Set Prod. No. ALX-850-014) increases the activity of rhsFasL approx. 50-fold.
Attention: Results using sFasL may differ from those obtained with agonistic antibodies!
 
Applications:ELISA
 
Application Notes:ELISA: binds to Fas receptor at 1-100 ng/ml.
 
Reconstitution:Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least 6 months after receipt when stored at -20°C.
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
 
Technical Info/Product Notes:Historical lots have shown that FasL kills Jurkat cells at concentrations of >10ng/ml in the absence of a cross-linker. Historical lots have also shown an ED50 of 50ng/ml (A20 cells).
 
522-001 AA
Activity Assay analysis: Inhibition of FasL, Soluble (human) (rec.) (Prod. No. ALX-522-001)-mediated lysis. Fas (human):Fc (human) (rec.) (Prod. No. ALX-522-002) exerts its inhibitory activity in a concentration range of 0.5-5μg/ml in the presence of the enhancer (1μg/ml).
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522-001 AA

Product Literature References

Role of the cell cycle in regression of the corpus luteum: S.M. Quirk, et al.; Reproduction 145, 161 (2013), Application(s): Death induction of bovine luteal cells, Abstract; Full Text
The Fas/FasL pathway impairs the alveolar fluid clearance in mouse lungs: R. Herrero, et al.; Am. J. Physiol. Lung Cell Mol. Physiol. 305, L377 (2013), Application(s): Death induction of primary mouse alveolar epithelial and endothelial cells, Abstract;
A novel TNFR1-triggered apoptosis pathway mediated by class IA PI3Ks in neutrophils: B. Geering, et al.; Blood 117, 5953 (2011), Application(s): Death induction of murine bone marrow neutrophils, Abstract; Full Text
Different Signaling Pathways Stimulate a Disintegrin and Metalloprotease-17 (ADAM17) in Neutrophils during Apoptosis and Activation: Y. Wang, et al.; J. Biol. Chem. 286, 38980 (2011), Application(s): Death induction of Jurkat cells, Abstract; Full Text
A role for cFLIP in B cell proliferation and stress MAPK regulation: H. Zhang, et al.; J. Immunol. 182, 207 (2009), Abstract; Full Text
Redox amplification of apoptosis by caspase-dependent cleavage of glutaredoxin 1 and S-glutathionylation of Fas: V. Anathy, et al.; J. Cell Biol. 184, 241 (2009), Application(s): Death induction of C10 cells, Fibroblasts and CD4+ T lymphocytes, Abstract; Full Text
Caspase-8 Cleaves Histone Deacetylase 7 and Abolishes Its Transcription Repressor Function: F.L. Scott, et al.; J. Biol. Chem. 283, 19499 (2008), Application(s): Death induction of primary mouse thymocytes, Abstract; Full Text
Loss of the BH3-only protein Bmf impairs B cell homeostasis and accelerates gamma irradiation-induced thymic lymphoma development: V. Labi, et al.; J. Exp. Med. 205, 641 (2008), Abstract; Full Text
CD4-Dependent Signaling Is Required for a Late Checkpoint during Th2 Development Associated with Resistance to Activation-Induced Cell Death: Z. Tatari-Calderone, et al.; J. Immunol. 175, 5629 (2005), Application(s): Death induction of murine T cells, Abstract; Full Text
Cytoskeleton-mediated death receptor and ligand concentration in lipid rafts forms apoptosis-promoting clusters in cancer chemotherapy: C. Gajate & F. Mollinedo; J. Biol. Chem. 280, 11641 (2005), Abstract; Full Text
Cathepsin-B-dependent apoptosis triggered by antithymocyte globulins: a novel mechanism of T-cell depletion: M. C. Michallet, et al.; Blood 102, 3719 (2004), Abstract;
The anti-apoptotic factor Bcl-2 can functionally substitute for the B cell survival but not for the marginal zone B cell differentiation activity of BAFF: A. Tardivel, et al.; Eur. J. Immunol. 34, 509 (2004), Abstract;
A second step of chemotaxis after transendothelial migration: keratinocytes undergoing apoptosis release IFN-gamma-inducible protein 10, monokine induced by IFN-gamma, and IFN-gamma-inducible alpha-chemoattractant for T cell chemotaxis toward epidermis in: S. Klunker, et al.; J. Immunol. 171, 1078 (2003), Abstract; Full Text
Theileria parva-Transformed T Cells Show Enhanced Resistance to Fas/Fas Ligand-Induced Apoptosis : P. Küenzi, et al.; J. Immunol. 171, 1224 (2003), Application(s): Death induction of Theileria parva-infected TpM(803) T cells, Abstract; Full Text
BCR engagement induces Fas resistance in primary B cells in the absence of functional Bruton's tyrosine kinase: J.R. Tumang, et al.; J. Immunol. 168, 2712 (2002), Abstract;
Hepatic natural killer cells exclusively kill splenic/blood natural killer-resistant tumor cells by the perforin/granzyme pathway: D. Vermijlen, et al.; J. Leukoc. Biol. 72, 668 (2002), Application(s): Death induction of P815 cells, Abstract; Full Text
Fas ligand overexpression on allograft endothelium inhibits inflammatory cell infiltration and transplant-associated intimal hyperplasia: M. Sata, et al.; J. Immunol. 166, 6964 (2001), Abstract;
Fas-associated death domain protein (FADD) and caspase-8 mediate up-regulation of c-Fos by Fas ligand and tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) via a FLICE inhibitory protein (FLIP)-regulated pathway: D. Siegmund, et al.; J. Biol. Chem. 276, 32585 (2001), Abstract; Full Text
Mechanisms underlying neuronal death induced by chromogranin A-activated microglia: J. Ciesielski-Treska, et al.; J. Biol. Chem. 276, 13113 (2001), Abstract; Full Text
T cell costimulation by the TNF ligand BAFF: B. Huard, et al.; J. Immunol. 167, 6225 (2001), Abstract;
Crosstalk Between Keratinocytes and T Lymphocytes via Fas/Fas Ligand Interaction: Modulation by Cytokines: R. Arnold, et al.; J. Immunol. 162, 7140 (1999), Application(s): Death induction of Jurkat cells, Abstract; Full Text
Fas-Mediated Suicide of Tumor-Reactive T Cells Following Activation by Specific Tumor: Selective Rescue by Caspase Inhibition: T.Z. Zaks, et al.; J. Immunol. 162, 3273 (1999), Application(s): Death induction of tumor-specific CD8+ T cells, Abstract; Full Text
Inhibition of Toxic Epidermal Necrolysis by Blockade of CD95 with Human Intravenous Immunoglobulin: I. Viard, et al.; Science 282, 490 (1998), Application(s): Death induction of human keratinocytes, Abstract;
Agonist antibody and Fas ligand mediate different sensitivity to death in the signaling pathways of Fas and cytoplasmic mutants: A.R. Thilenius, et al.; Eur. J. Immunol. 27, 1108 (1997), Abstract;
Characterization of Fas (Apo-1, CD95)-Fas ligand interaction: P. Schneider, et al.; J. Biol. Chem. 272, 18827 (1997), Abstract; Full Text

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FasL, soluble (human) (recombinant) set 

Produced in HEK 293 cells. The extracellular domain of human FasL (APO-1L; CD95L; CD178) (aa 103-281) is fused at the N-terminus to a linker peptide (26 aa) and a FLAG®-tag. Glycosylation of FasL, Soluble (human) (recombinant) is similar to natural human FasL., ≥95% (SDS-PAGE), FUNC | Print as PDF
 
ALX-850-014-KI02 1 Set 591.00 USD
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