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Nerve growth factor 7S (mouse)

 
ALX-521-008-C100 100 µg 333.00 USD
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Product Specification

Alternative Name:NGF 7S
 
MW:~130kDa.
 
Source:Isolated from mouse submaxillary glands by a modification of the procedure described by T. Darling, et al.; Cell Culture Meth. Mol. Cell Biol. 4, 79 (1984).
 
UniProt ID:P01139
 
Quantity:Determined by Lowry and Pierce method.
 
Formulation:Lyophilized.
 
Purity:≥97% (SDS-PAGE)
 
Biological Activity:Bioassayed for neurotrophic activity using the rat pheochromocytoma cell PC12 cultures over a period of 7-14 days. Neurotrophic activity is in the range of 0.5-50ng/ml. Recommended concentration to be used in vitro for maintenance of sympathetic and immature sensory nerve cells is 50-100ng/ml medium.
 
Reconstitution:Dilute in sterile water or DMEM to a concentration of at least 10µg/ml and store at -80°C before use. Medium containing NGF cannot be reused due to NGF degradation.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Use/Stability:Stable for 2-3 weeks when stored at room temperature. After reconstitution, store for up to one week at +4°C and for 4-6 weeks at -80°C.
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
 

Product Literature References

Neurobiology. Tracking neurotrophin function: A.M. Davies; Nature 368, 193 (1994), (Review), Abstract;
Neurotrophic factors enter the clinic: M. Barinaga; Science 264, 772 (1994), (Review), Abstract;
Neurotrophic factors: two are better than one: R. Nishi; Science 265, 1052 (1994), (Review), Abstract;
Role of neurotrophins in mouse neuronal development: R. Klein; FASEB J. 8, 738 (1994), (Review), Abstract;
The neurotrophins and their receptors: structure, function, and neuropathology: L.M. Maness, et al.; Neurosci. & Biobehav. Rev. 18, 143 (1994), (Review), Abstract;
Towards a comprehensive understanding of the trophic support of motoneurons: H. Thoenen, et al.; C. R. Acad. Sci. III 316, 1158 (1993), (Review), Abstract;
The changing scene of neurotrophic factors: H. Thoenen; TINS 14, 165 (1991), (Review), Abstract;
The isolation of the mouse nerve growth factor protein in a high molecular weight form: S. Varon, et al.; Biochemistry 6, 2202 (1967), Abstract;
Protein measurement with the Folin phenol reagent: O.H. Lowry, et al.; J. Biol. Chem. 193, 265 (1951), Abstract;

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