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Nerve growth factor 2.5S (mouse), (grade I)

ALX-521-006-C010 10 µg 176.00 USD
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Product Specification

Alternative Name:NGF 2.5S
MW:~26kDa; homodimer.
Source:Isolated from mouse submaxillary glands by a modification of the procedure described by T. Darling, et al.; Cell Culture Meth. Mol. Cell Biol. 4, 79 (1984).
UniProt ID:P01139
Quantity:mNGF 2.5S concentration is estimated spectroscopically from its extinction coefficient and amino acid analysis.
Purity:≥98% (SDS-PAGE)
Biological Activity:mNGF 2.5S was bioassayed for neurotrophic activity using the rat pheochromocytoma cell PC12 cultures over a period of 7 - 14 days. The neurotrophic activity is in the range of 0.5-50ng/ml. The recommended concentration to be used in vitro for maintenance of sympathetic and sensory nerve cultures is 50ng/ml medium.
Reconstitution:Reconstitute in sterile water or DMEM at a concentration of at least 10µg/ml and store at -80°C before use. Medium with mNGF cannot be reused due to mNGF degradation.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Use/Stability:Stable for 2-3 weeks when stored at room temperature. After reconstitution, stable for up to 1 week when stored at +4°C and for 4-6 weeks at -80°C.
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.

Product Literature References

Neurobiology. Tracking neurotrophin function: A.M. Davies; Nature 368, 193 (1994), (Review), Abstract;
Neurotrophic factors enter the clinic: M. Barinaga; Science 264, 772 (1994), (Review), Abstract;
Neurotrophic factors: two are better than one: R. Nishi; Science 265, 1052 (1994), (Review), Abstract;
Role of neurotrophins in mouse neuronal development: R. Klein; FASEB J. 8, 738 (1994), (Review), Abstract;
The neurotrophins and their receptors: structure, function, and neuropathology: L.M. Maness, et al.; Neurosci. & Biobehav. Rev. 18, 143 (1994), (Review), Abstract;
Towards a comprehensive understanding of the trophic support of motoneurons: H. Thoenen, et al.; C. R. Acad. Sci. III 316, 1158 (1993), (Review), Abstract;
The changing scene of neurotrophic factors: H. Thoenen; TINS 14, 165 (1991), (Review), Abstract;
Nerve growth factor: R.A. Bradshaw; Annu. Rev. Biochem. 47, 191 (1978), Abstract;

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