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β-Nerve growth factor (human), (recombinant)

 
ALX-521-005-C005 5 µg 117.00 USD
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Product Specification

Alternative Name:β-NGF
 
MW:~27kDa (dimer).
 
Source:Produced in E. coli. Dimer of two identical subunits (119 aa) linked together by very strong hydrophobic interactions.
 
CAS:86923-98-0
 
UniProt ID:P01138
 
Quantity:5µg (determined by amino acid analysis).
 
Formulation:Lyophilized. No additives.
 
Purity:≥98% (HPLC)
 
Biological Activity:Neurotrophic activity was bioassayed using rat pheochromocytoma PC12 cell cultures over a period of 5-14 days. Activity is in the range  of 1-50ng/ml.
 
Reconstitution:Reconstitue in water or DMEM to a final concentration of at least 10µg/ml and store at -80°C before use.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Use/Stability:Lyophilized protein is stable for 2-3 weeks when stored at room temperature and for 6-9 months when stored at -20°C.Reconstituted protein is stable for up to 1 week when stored at +4°C and for 4-6 weeks when stored at -80°C.
 
Handling:Avoid freeze/thaw cycles.
 
Scientific Background:Human β-NGF is responsible for survival, differentiation and maturation of sympathetic and sensory neurons.
 
521-005
Figure: Neurite outgrowth in PC12 cells. Method: PC12 cells were grown on collagen coated 24-well plates in DMEM medium supplemented with 6% fetal calf serum and 6% horse serum for 24 hours. β-Nerve Growth Factor (human) (rec.) (Prod. No. ALX-521-005) was addded at the indicated concentrations. The culture media was changed every 2-3 days. The development of neurites over a period of 5 days was visualized by methylene blue staining.
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General Literature References

Neurobiology. Tracking neurotrophin function: A.M. Davies; Nature 368, 193 (1994), (Review), Abstract;
Neurotrophic factors enter the clinic: M. Barinaga; Science 264, 772 (1994), (Review), Abstract;
Neurotrophic factors: two are better than one  : R. Nishi; Science 265, 1052 (1994), (Review), Abstract;
Role of neurotrophins in mouse neuronal development: R. Klein; FASEB J. 8, 738 (1994), (Review), Abstract;
The neurotrophins and their receptors: structure, function, and neuropathology: L.M. Maness, et al.; Neurosci. & Biobehav. Rev. 18, 143 (1994), (Review), Abstract;
Towards a comprehensive understanding of the trophic support of motoneurons: H. Thoenen, et al.; C.R. Acad. Sci. III 316, 1158 (1993), (Review), Abstract;
The changing scene of neurotrophic factors : H. Thoenen; TINS 14, 165 (1991), (Review), Abstract;

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