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KillerTRAIL™ Storage and Dilution Buffer

 
ALX-505-005-R500 500 µl 170.00 USD
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Storage and dilution buffer for use with the following products:
KillerTRAIL™, Soluble (human) (recombinant) (Prod. No. ALX-201-073)
SuperKillerTRAIL™, Soluble (human) (recombinant) (Prod. No. ALX-201-115)
SuperKillerTRAIL™, Soluble (mouse) (recombinant) (Prod. No. ALX-201-130)
Useful for prediluting the above mentioned products before preparing aliquots or performing experiments. Predilution with KillerTRAIL™ Storage and Dilution Buffer allows for easier handling of small working concentrations (sometimes only 0.1µl of the KillerTRAIL™ or SuperKillerTRAIL™ stock solution is necessary because of their high activity). Predilution with KillerTRAIL™ Storage and Dilution Buffer instead of medium or PBS prevents loss of biological activity. Can also be used as negative control.

Product Specification

Formulation:20mM HEPES, pH 7.4, containing 300mM NaCl, 0.01% Tween 20, 1% sucrose and 1mM DTT.
 
Purity Detail:Sterile filtered (0.22 µm).
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-80°C
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
 
Technical Info/Product Notes:Prepare a 1:5 dilution of KillerTRAIL™ or SuperKillerTRAIL™ with KillerTRAIL™ Storage and Dilution Buffer. For immediate use, keep prediluted KillerTRAIL™ or SuperKillerTRAIL™ on ice until adding to cells.
 

Product Literature References

Bortezomib Sensitizes Malignant Human Glioma Cells to TRAIL, Mediated by Inhibition of the NF-κB Signaling Pathway: E.P. Jane, et al.; Mol. Cancer Ther. 10, 198 (2011), Application(s): Death induction on a panel of 8 glioma cell lines, Abstract; Full Text

Related Products

KillerTRAIL™ (soluble) (human), (recombinant) 

Enhanced ligand that utilizes the proprietary Killer™ linker peptide that promotes stability.
Produced in E. coli. The extracellular domain of human TRAIL (aa 95-281) is fused at the N-terminus to a His-tag and a linker peptide., ≥99% (SDS-PAGE, MS analysis) | Print as PDF
 
ALX-201-073-C020 20 µg 435.00 USD
 
ALX-201-073-3020 3x20 µg 895.00 USD
Do you need bulk/larger quantities?
 

SuperKillerTRAIL™ (soluble) (mouse), (recombinant) 

Produced in E. coli. The extracellular domain of mouse TRAIL (aa 99-291) is fused at the N-terminus to a His-tag and a linker peptide. The active multimeric conformation is stabilized by an inserted mutation allowing an additional CC-bridge., ≥95% (SDS-PAGE) | Print as PDF
 
ALX-201-130-C020 20 µg 412.00 USD
 
ALX-201-130-3020 3x20 µg 895.00 USD
Do you need bulk/larger quantities?
 

SuperKillerTRAIL™ (soluble) (human), (recombinant) 

Enhanced ligand with improved stability providing significantly enhanced immune activation.
Produced in E. coli. The extracellular domain of human TRAIL (aa 95-281) is fused at the N-terminus to a His-tag and a linker peptide. The active multimeric conformation is stabilized by an inserted mutation allowing an additional CC-bridge., ≥98% (SDS-PAGE). MS analysis, <1% impurity (mainly Hsp70 protein from E. coli) | Print as PDF
 
ALX-201-115-C010 10 µg 435.00 USD
 
ALX-201-115-3010 3x10 µg 922.00 USD
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