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Hecameg®

Glucose-based detergent
 
ALX-500-003-G001 1 g Inquire for pricing
 
ALX-500-003-G005 5 g 240.00 USD
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Non-ionic, glucose based very mild detergent for membrane protein purification.

Product Specification

Alternative Name:6-O-(N-Heptylcarbamoyl)-methyl-α-D-glucopyranoside
 
Formula:C15H29NO7
 
MW:335.4
 
CAS:115457-83-5
 
Purity:≥98% (HPLC)
 
Appearance:White to off-white crytalline solid.
 
Solubility:Soluble in water (>100mg/ml at +4°C).
 
Application Notes:Extraction, purification, stabilization of proteins (recombinant or natural proteins).
Surfactant for chromatography, electrophoresis and ELISA analysis.
Extraction of other biomolecules (DNA and RNA) from proteous samples.
Study of protein structure; Reconstitution or crystallisation of membrane proteins, enzymes or antigens; Liposomes preparation.
Sanitization of chromatography columns.
 
Shipping:Ambient
 
Long Term Storage:+4°C
 
Technical Info/Product Notes:Hecameg is a synthetic, well defined high quality detergent, providing consistent and reliable results.
The critical micellar concentration is CMC= 19.5mM, which allow it’s easy removal by dialysis.
An effective surfactant detergent that preserves proteins, dissociates aggregated proteins, helps breaking biological membranes without denaturing proteins, enzymes or antigens, because it is non charged.
Hecameg does not interfere with their biological activity, as shown for more than hundred enzymes, antigens and receptors.
It was used for extraction of proteins from Chromaffin granules of mammalian cells, at 4% (Hodel 1994), photosystem II core complex (Kouimtzoglou 1994), and Heparan Sulfate ProteoGlycan (Kiran 1994).
50mM Hecameg with EDTA, was found to give the highest yields of active lectinic factor (involved in yeast flocculation) in comparison to other detergents (El-Behhari 1998).
It was used at several steps of the isolation of Bf6 cytochrome from tylakoids of Chlamydomonas alga (Pierre 1995): cells were suspended in saline buffer with 25mM Hecameg, and centrifuged. The supernatant was fractionated by centrifugation in 10-30% w/w sucrose density gradient in presence of 20mM Hecameg and 0.1g/l egg phosphatidyl choline. Lastly, affinity chromatography was performed on hydroxylapatite, eluting with 20mM Hecameg plus 0.1g/l egg phosphatidyl choline.

Purification
Addition of 0.05% w/v Hecameg enhances recovery of material from hydroxyapatite and Q-Sepharose columns, and decreases elution volumes (Gerngross 1994).

Analysis
Hecameg produced the best diffracting crystals of Cytochrome bc1 complex (Lee 1995), in comparison with octyl-b-D-glucopyranoside, MEGA -9, n-octanoylsucrose and octyl-b-D-maltopyranoside. This was attribuated to a better stability of proteins.
It was shown to be effective for reconstitution procedures in which detergents must be removed by dialysis, and for the lipid solubilization and uptake of vesicle contents at concentration well below the solubilizing range (Begona Ruiz 1994)
 
ALX-500-003
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ALX-500-003

Product Literature References

The ABC transporter BmrA from Bacillus subtilis is a functional dimer when in a detergent-solubilized state: S. Ravaud, et al.; Biochem. J. 395, 345 (2006), Abstract; Full Text
Comparative extraction procedures for a galactose-specific lectin involved in flocculation of Kluyveromyces lactis strains: M. El-Behhari, et al.; Appl. Microbiol. Biotechnol. 49, 16 (1998), Abstract;
Dimer to monomer conversion of the cytochrome b6 f complex. Causes and consequences: C. Breyton, et al.; J. Biol. Chem. 272, 21892 (1997), Abstract; Full Text
On the presence and role of a molecule of chlorophyll a in the cytochrome b6 f complex: Y. Pierre, et al.; J. Biol. Chem. 272, 21901 (1997), Abstract; Full Text
Preliminary Cryocristallographic Study of the Mitochondrial Cytochrome bc1 complex: Improved Crystallization and Flash-cooling of a Large Membrane Protein: J.W. Lee, et al.; J. Mol. Biol. 252, 15 (1995), Abstract;
Purification and Characterization of the Cytochrom b6f Complex from Chlamydomonas reinhardtii: Y. Pierre, et al.; J. Biol. Chem. 270, 29342 (1995), Abstract; Full Text
An assessment of the biochemical applications of the non-ionic surfactant HECAMEG(R): M. Begona Ruiz, et al.; Biochim. Biophys. Acta 1193, 301 (1994), Abstract;
In Chromaffin Cells, the Mammalian Sec1p Homologue Is a Syntaxin 1A-binding Protein Associated with Chromaffin Granules: A. Hodel, et al.; J. Biol. Chem. 269, 8623 (1994), Abstract; Full Text
Overexpression and Purification of the Soluble Polyhydroxyalkanoate Synthase from Alcaligenes eutrophus.: Evidence for a Required Posttranslational Modification for Catalytic Activity: T.U. Gerngross, et al.; Biochemistry 33, 9311 (1994), Abstract;
Purification and Characterization of Adipocyte Heparan Sulfate Proteoglycans with Affinity for Lipoprotein Lipase: K.B. Misra, et al.; J. Biol. Chem. 269, 23838 (1994), Abstract; Full Text
Purification and Characterization of Protein H, the Major Porin of Pasteurella multocida: G. Chevalier, et al.; J. Bacteriol. 175, 266 (1993), Abstract;
Ultrasonic Absorption Studies of Surfactant Exchange between Micelles and Bulk Phase in Aqueous Micellar Solutions of Nonionic Surfactants with a Short Alkyl Chain. 3.Surfactants with a Sugar Head Gp: M. Frindi, et al.; J. Physiol. Chem. 96, 8137 (1992),
Synthesis and Characterization of 6-O-(N-Heptylcarbamoyl)-methyl-alpha-D-glucopyranoside, a New Surfactant for Membrane Studies: D. Plusquellec, et al.; Anal. Biochem. 179, 145 (1989), Abstract;

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