Analog of CPH (Prod. No. ALX-430-078) with higher cell permeability and 3-fold better reactivity with superoxide. Has been used to study intracellular production of superoxide radicals in plasma, endothelial cells and isolated hearts.
Product Details
| Alternative Name: | 1-Hydroxy-3-methoxycarbonyl-2,2,5,5-tetramethylpyrrolidine . HCl |
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| Formula: | C10H19NO3 . HCl |
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| MW: | 201.3 . 36.5 |
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| Purity: | ≥99% (EPR) |
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| Appearance: | White to light-yellow crystalline powder. |
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| Solubility: | Soluble in DMSO, 100% ethanol, methanol or water. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Prepare fresh solutions daily before use and store 4-6 hours on ice under a nitrogen/argon flow.
Oxygen will lead to oxidation and therefore it is strongly recommended to dissolve the probe in water and not in organic solvents such as DMSO, ethanol or methanol. Oxygen is 10 times more soluble in the latter solvents. Freezing solutions will not lead to better results, because mixing with air after melting the probe solutions will results in faster oxidation.
Also, it is strongly recommended to store the product and its solutions in glass vials. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
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Reactive oxygen species regulatory mechanisms associated with rapid response of MC3T3-E1 cells for vibration stress: L. Zhang, et al.; Biochem. Biophys. Res. Commun.
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Selective inhibition of deactivated mitochondrial complex I by biguanides: S. Matsuzaki, et al.; Biochemistry
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EPR detection of cellular and mitochondrial superoxide using cyclic hydroxylamines: S.I. Dikalov, et al.; Free Rad. Res.
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Measurement of reactive oxygen species in cardiovascular studies: S. Dikalov, et al.; Hypertension
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Atrial fibrillation increases production of superoxide by the left atrium and left atrial appendage: role of the NADPH and xanthine oxidases: S.C. Dudley, Jr., et al.; Circulation
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Detection of superoxide with new cyclic hydroxylamine CMH in plasma, cells and isolated heart: B. Fink & S. Dikalov; Free Radical Biol. Med. 33, S366 (2002),
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