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United States 

CMH . hydrochloride

Spin probe reagent
ALX-430-117-M010 10 mg 78.00 USD
ALX-430-117-M050 50 mg 234.00 USD
ALX-430-117-M250 250 mg 541.00 USD
Do you need bulk/larger quantities?
Analog of CPH (Prod. No. ALX-430-078) with higher cell permeability and 3-fold better reactivity with superoxide. Has been used to study intracellular production of superoxide radicals in plasma, endothelial cells and isolated hearts.

Product Specification

Alternative Name:1-Hydroxy-3-methoxycarbonyl-2,2,5,5-tetramethylpyrrolidine . HCl
Formula:C10H19NO3 . HCl
MW:201.3 . 36.5
Purity:≥99% (EPR)
Appearance:White to light-yellow crystalline powder.
Solubility:Soluble in DMSO, 100% ethanol, methanol or water.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Use/Stability:Prepare fresh solutions daily before use and store 4-6 hours on ice under a nitrogen/argon flow.
Oxygen will lead to oxidation and therefore it is strongly recommended to dissolve the probe in water and not in organic solvents such as DMSO, ethanol or methanol. Oxygen is 10 times more soluble in the latter solvents. Freezing solutions will not lead to better results, because mixing with air after melting the probe solutions will results in faster oxidation.
Also, it is strongly recommended to store the product and its solutions in glass vials.
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Product Literature References

Reactive oxygen species regulatory mechanisms associated with rapid response of MC3T3-E1 cells for vibration stress: L. Zhang, et al.; Biochem. Biophys. Res. Commun. 470, 510 (2016), Application(s): Injected into cells for intracellular ROS determination, Abstract;
Bone Morphogenic Protein 4 Mediates NOX1-dependent eNOS Uncoupling, Endothelial Dysfunction and COX2 Induction in Type 2 Diabetes Mellitus: J.Y. Youn, et al.; Mol. Endocrinol. 29, 1123 (2015), Application(s): Electron Spin Resonance, Abstract; Full Text
Selective inhibition of deactivated mitochondrial complex I by biguanides: S. Matsuzaki, et al.; Biochemistry 54, 2011 (2015), Application(s): Electron Paramagnetic Resonance Spin Trapping, Abstract; Full Text
Sex-based differential regulation of oxidative stress in the vasculature by nitric oxide: R.C. Morales, et al.; Redox Biol. 14C, 226 (2015), Application(s): Cell Culture, Electron Paramagnetic Resonance Analysis, Abstract;
Role of Carnitine Acetyl Transferase in Regulation of Nitric Oxide Signaling in Pulmonary Arterial Endothelial Cells: S. Sharma, et al.; Int. J. Mol. Sci. 14, 255 (2013), Application(s): Electron paramagnetic resonance (EPR) spectroscopy, Abstract; Full Text
EPR detection of cellular and mitochondrial superoxide using cyclic hydroxylamines: S.I. Dikalov, et al.; Free Rad. Res. 45, 417 (2011), Abstract;
Measurement of reactive oxygen species in cardiovascular studies: S. Dikalov, et al.; Hypertension 49, 717 (2007), Abstract;
Production of extracellular superoxide by human lymphoblast cell lines: comparison of electron spin resonance techniques and cytochrome C reduction assay: S.I. Dikalov, et al.; Biochem. Pharmacol. 73, 972 (2007), Abstract; Full Text
Atrial fibrillation increases production of superoxide by the left atrium and left atrial appendage: role of the NADPH and xanthine oxidases: S.C. Dudley, Jr., et al.; Circulation 112, 1266 (2005), Abstract;
Interactions of peroxynitrite with uric acid in the presence of ascorbate and thiols: implications for uncoupling endothelial nitric oxide synthase: N. Kuzkaya, et al.; Biochem. Pharmacol. 70, 343 (2005), Abstract;
Nox1 overexpression potentiates angiotensin II-induced hypertension and vascular smooth muscle hypertrophy in transgenic mice: A. Dikalova, et al.; Circulation 112, 2668 (2005), Abstract;
Oscillatory shear stress stimulates endothelial production of O2- from p47phox-dependent NAD(P)H oxidases, leading to monocyte adhesion: J. Hwang, et al.; J. Biol. Chem. 278, 47291 (2003), Abstract; Full Text
Detection of superoxide with new cyclic hydroxylamine CMH in plasma, cells and isolated heart: B. Fink & S. Dikalov; Free Radical Biol. Med. 33, S366 (2002),

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CPH . hydrochloride 

Spin probe reagent
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ALX-430-078-M010 10 mg 72.00 USD
ALX-430-078-M050 50 mg 234.00 USD
ALX-430-078-M250 250 mg 499.00 USD
Do you need bulk/larger quantities?

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