Effective, non-cell permeable and non-toxic analog of CPH for the detection of superoxide radicals. Resistant against reduction of vitamin C. For cell permeable analog see CPH (Prod. No. ALX-430-078).
Product Details
| Alternative Name: | 1-Hydroxy-4-phosphono-oxy-2,2,6,6-tetramethylpiperidine . HCl |
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| Formula: | C9H20NO5P . HCl |
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| MW: | 253.2 . 36.5 |
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| Purity: | ≥99% (EPR) |
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| Purity Detail: | ≤0.1% paramagnetic impurities (EPR) |
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| Appearance: | White to off-white solid. |
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| Solubility: | Soluble in DMSO, 100% ethanol, methanol or water. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Technical Info/Product Notes: | The stabilized acid form of PPH is not well soluble at neutral pH (~0.1-0.01%). It is recommended to adjust pH to 7.4 for higher solubility. Dissolve PPH in oxygen-free buffers at neutral pH and use 1M NaOH to adjust pH to 7.4. Solubility is 1% in water, 0.8% in methanol or 0.5% in ethanol at pH 7.4. Solubility is 3% in DMSO at pH 7.4 (first add NaOH to PPH crystals then DMSO). Higher concentrations require further pH adjustment using NaOH. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
EPR detection of cellular and mitochondrial superoxide using cyclic hydroxylamines: S.I. Dikalov, et al.; Free Rad. Res.
45, 417 (2011),
Abstract;
Molecular Mechanisms of Angiotensin II Mediated Mitochondrial Dysfunction. Linking Mitochondrial Oxidative Damage and Vascular Endothelial Dysfunction: A.K. Doughan, et al.; Circ. Res.
102, 488 (2008),
Abstract;
Rotenone model of Parkinson disease: multiple brain mitochondria dysfunctions after short term systemic rotenone intoxication: A. Panov, et al.; J. Biol. Chem.
280, 42026 (2005),
Abstract;
Noninvasive diagnostic tool for inflammation-induced oxidative stress using electron spin resonance spectroscopy and an extracellular cyclic hydroxylamine: S.I. Dikalov, et al.; Arch. Biochem. Biophys.
402, 218 (2002),
Abstract;
A new approach for extracellular spin trapping of nitroglycerin-induced superoxide radicals both in vitro and in vivo: B. Fink, et al.; Free Radic. Biol. Med.
28, 121 (2000),
Abstract;
Detection of superoxide radicals and peroxynitrite by 1-hydroxy-4- phosphonooxy-2,2,6,6-tetramethylpiperidine: quantification of extracellular superoxide radicals formation: S. Dikalov, et al.; BBRC
248, 211 (1998),
Abstract;
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