Replaces Prod. #: BML-EI156
- Model apoptosis inducer
- Potent cell-permeable inhibitor of protein kinases
- Highly cited
Staurosporine is the reference agent for apoptosis induction (1µM in CHO cells). Staurosporine binds to the ATP binding site and inhibits a variety of protein kinases including protein kinase C (PKC), CDK1/cyclin B (IC50~5nM), CDK2/cyclin A (IC50=7nM), CDK4/cyclin D (IC50=3-10µM), CDK5/p25 (IC50=4nM), GSK-3β (IC50=15nM), and Pim-1 kinase (IC50=10nM). Staurosporine does not inhibit PKC-ζ. Staurosporine also inhibits topoisomerase II directly by blocking transfer of phosphodiester bonds from DNA to active site tyrosine. Other than apoptosis and cytotoxicity, some of the biological effects of staurosporine include regulation of eNOS gene expression and relaxation of smooth mucles.
Product Details
| Alternative Name: | Antibiotic AM-2282 |
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| Formula: | C28H26N4O3 |
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| MW: | 466.5 |
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| Source: | Isolated from Streptomyces staurosporeus. |
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| CAS: | 62996-74-1 |
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| MI: | 14: 8802 |
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| RTECS: | KC655000 |
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| Purity: | ≥99% (HPLC) |
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| Appearance: | Off-white to green powder. |
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| Solubility: | Soluble in DMF (25mg/ml), DMSO (25mg/ml), or ethyl acetate. Only slightly soluble in chloroform and methanol. Insoluble in water. |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | +4°C |
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| Use/Stability: | Stable for at least 2 years after receipt when stored +4°C. |
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| Handling: | Protect from light and moisture. Store under inert gas. |
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| Technical Info/Product Notes: | Replacement for ADI-HPK-112 |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
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Caspase-8 activation and bid cleavage contribute to MCF7 cellular execution in a caspase-3-dependent manner during staurosporine-mediated apoptosis: D. Tang, et al.; J. Biol. Chem.
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Changes in mitochondrial membrane potential during staurosporine- induced apoptosis in Jurkat cells: J.L. Scarlett, et al.; FEBS Lett.
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Dissociation of staurosporine-induced apoptosis from G2-M arrest in SW620 human colonic carcinoma cells: initiation of the apoptotic cascade is associated with elevation of the mitochondrial membrane potential (deltapsim): B.G. Heerdt, et al.; Cancer Res.
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