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Convulxin

Heterodimeric C-type lectin
 
ALX-350-100-C050 50 µg Inquire for pricing
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Heterodimeric C-type lectin. Activates mammalian platelets via binding and clustering of p62/GPVI-receptors under physiological conditions.

Product Specification

MW:~84kDa.
 
Source:Isolated from Crotalus durissus terrificus snake venom.
 
CAS:37206-04-5
 
UniProt ID:O93426
 
Formulation:Lyophilized.
 
Purity Detail:Partially purified by multi-step chromatography.
 
Activity:<50ng/ml (minimal concentration to induce maximum activation of washed human platelets).
 
Application Notes:Can be used for studies on platelet receptors.
 
Solubility:Soluble in organic buffer (HEPES).
 
Reconstitution:Reconstitute in 10mM HEPES buffer, pH 7.2. (We recommend adding a carrier protein such as BSA to the reconstitution buffer and any subsequent dilution buffers to prevent absorption on plastic surfaces.)
 
Shipping:Ambient
 
Long Term Storage:-20°C
 
Use/Stability:Stock solutions are stable for 8 hours at +20°C, for 2 days at +4°C or for 1 month when stored at -80°C.
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
 
Protocol:Assay performed in aggregometer:
  1. Incubate 485µl platelets suspension (5x108 platelets/ml) for 1-2 minutes at 37°C until base line is stable.
  2. Add 5µl 200mM CaCl2.
  3. Add 5µl 200mM MgCl2.
  4. Wait until baseline is stable.
  5. Add 5µl collagen solution (0.5mg/ml) for positive control OR add 5µl convulxin (0.1µg/ml in HEPES).
  6. Measure aggregation at 37°C.
 

Product Literature References

Establishment of immunoassay for platelet-derived soluble glycoprotein VI, a novel platelet marker: K. Naitoh, et al.; J. Immunol. Methods 418, 52 (2015), Application(s): Cell Culture, Assay, Abstract;
Peroxiredoxin II is an antioxidant enzyme that negatively regulates collagen-stimulated platelet function: J. Y. Jang, et al.; J. Biol. Chem. 290, 11432 (2015), Application(s): Cell Culture , Abstract; Full Text
Resveratrol inhibits collagen-induced platelet stimulation through suppressing NADPH oxidase and oxidative inactivation of SH2 domain-containing protein tyrosine phosphatase-2: J.Y. Jang, et al.; Free Radic. Biol. Med. 89, 842 (2015), Application(s): Cell culture, Flow Cytometry, Abstract;
Mice Lacking the SLAM Family Member CD84 Display Unaltered Platelet Function in Hemostasis and Thrombosis: S. Hofmann, et al.; PLoS One 9, e11530 (2014), Application(s): Flow Cytometry, Abstract; Full Text
Paxillin is an intrinsic negative regulator of platelet activation in mice: A. Sakata, et al.; Thromb. J. 12, 1 (2014), Application(s): Cell Culture, Abstract; Full Text
Platelet production proceeds independently of the intrinsic and extrinsic apoptosis pathways: E.C. Josefsson, et al.; Nat. Commun. 5, 3455 (2014), Application(s): Cell Culture, Abstract;
Only severe thrombocytopenia results in bleeding and defective thrombus formation in mice: M. Morowski, et al.; Blood 121, 4938 (2013), Application(s): Flow Cytometry, Abstract; Full Text
Hyperglycemia-induced cerebral hematoma expansion is mediated by plasma kallikrein: J. Liu, et al.; Nat. Med. 17, 206 (2011), Abstract;
Diminished contact-dependent reinforcement of Syk activation underlies impaired thrombus growth in mice lacking Semaphorin 4D: K.M. Wannemacher, et al.; Blood 116, 5707 (2010), Abstract;
The human megakaryocytic cell line UT-7/TPO expresses functional platelet agonist signals mediated through GPVI and thromboxane receptor: T. Kawaguchi, et al.; Cell Biol. Int. 34, 943 (2010), Abstract;
Molecular priming of Lyn by GPVI enables an immune receptor to adopt a hemostatic role: A.A. Schmaier, et al.; PNAS USA 106, 21167 (2009), Abstract; Full Text
Structure of the snake-venom toxin convulxin: T. Batuwangala, et al.; Acta Crystallogr. D. Biol. Crystallogr. 60, 46 (2004), Abstract;
Convulxin binds to native, human glycoprotein Ib alpha: S. Kanaji, et al.; J. Biol. Chem. 278, 39452 (2003), Abstract;
Collagen, convulxin, and thrombin stimulate aggregation-independent tyrosine phosphorylation of CD31 in platelets. Evidence for the involvement of Src family kinases: M. Cicmil, et al.; J. Biol. Chem. 275, 27339 (2000), Abstract;
Convulxin-induced platelet adhesion and aggregation: involvement of glycoproteins VI and IaIIa: M. Jandrot-Perrus, et al.; Platelets 9, 207 (1998), Abstract;
Platelet activation and signal transduction by convulxin, a C-type lectin from Crotalus durissus terrificus (tropical rattlesnake) venom via the p62/GPVI collagen receptor: J. Polgar, et al.; J. Biol. Chem. 272, 13576 (1997), Abstract;
Convulxin, a new toxin from the venom of the South American rattlesnake Crotalus durissus terrificus: J. Prado-Franceschi & O.V. Brazil; Toxicon 19, 875 (1981), Abstract;

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