Cell permeable derivative of Prod. No. ALX-307-061.
|Identity:||Identity determined by 1H-NMR, 31P-NMR and MS.|
|Solubility:||Soluble in toluene and DMSO.|
|Shipping:||Shipped on Blue Ice|
|Long Term Storage:||-20°C|
|Use/Stability:||Do not freeze mixtures of Pluronic®, DMSO and inositolphosphates!|
|Handling:||Protect from moisture.|
|Technical Info/Product Notes:||Includes 1 vial of 10% Pluronic® (F127) solution in DMSO.|
|Protocol:||Recommendations on application (Cuvette Experiments)|
- Prepare a 100mM stock solution by adding 20µl of DMSO to 1µmol of inositol product.
- In a fresh vial mix 1µl of the stock solution and 2µl of Pluronic® as supplied.
- Add 500µl of your cell suspension and mix carefully.
- Add this mixture to the remaining cell suspension (~500µl) and mix carefully.
Note: Cell suspension should not contain more than 1% of DMSO.
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Product Literature References
myo-inositol 3,4,5,6-tetrakisphosphate inhibits an apical calcium-activated chloride conductance in polarized monolayers of a cystic fibrosis cell line
: M.A. Carew, et al.; J. Biol. Chem. 275
, 26906 (2000), Abstract
; Full Text
Long-term uncoupling of chloride secretion from intracellular calcium levels by Ins(3,4,5,6)P4
: M. Vajanaphanich, et al.; Nature 371
, 711 (1994), Abstract