trans-Ned-19



ALX-270-503-MC05	0.5 mg	181.00 USD

ALX-270-503-M001	1 mg	289.00 USD

Cell permeable, selective and potent inhibitor of nicotinic acid adenine dinucleotide phosphate (NAADP) receptor in mammalian cells and sea urchin eggs. Blocks NAADP signaling. Inhibits Ca²⁺release: trans-Ned-19 (IC₅₀= 6nM); cis-Ned-19 (IC₅₀= 800nM). [³²P] NAADP binding: trans-Ned-19 (IC₅₀= 0.4nM); cis-Ned-19 (IC₅₀= 15µM). Inhibits Ca²⁺ release from lysosomes. Noncompetitive antagonist.

Alternative Name:	1-(3-((4-(2-Fluorophenyl)piperazin-1-yl)methyl)-4-methoxyphenyl)-2,3,4,9-tetrahydro-1H-pyrido[3,4-b]indole-3-carboxylic acid

Identity:	Identity determined by ¹H-NMR, ¹³C-NMR and MS.

Formula:	C₃₀H₃₂FN₄O₃

MW:	515.6

Purity:	≥97% (HPLC)

Appearance:	White to off-white powder.

Source/Host:	Synthetic

Solubility:	Soluble in DMSO or 100% ethanol.

Long Term Storage:	-20°C

Handling:	Protect from light.

Miscellaneous/General:	NAADP (nicotinic acid adenine dinucleotide phosphate) is an emerging Ca2⁺ releasing second messenger in several mammalian tissues including pancreatic, brain and cardiac tissue.

Background / Technical Information:	Fluorescence of Ned-19 is compatible with visualization of the NAADP receptor in intact cells via confocal microscopy using an ultraviolet Argon ion laser (excitation 351 and 365 nm).

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Product Literature References

NAADP regulates human platelet function: C.H. Coxon, et al.; Biochem J. 441, 435 (2012), Abstract; Full Text

Actions of the NAADP Antagonist Trans-Ned19 in Cardiac Ventricular Myocytes: L. Elson, et al.; Biophys. J. 100, 81a (2011), Abstract;

Leucine-rich repeat kinase 2 regulates autophagy through a calcium-dependent pathway involving NAADP: P. Gomez-Suaga, et al.; Hum. Mol. Genet. in press, (2011), Abstract; Full Text

NAADP links histamine H1 receptors to secretion of von Willebrand factor in human endothelial cells: B. Esposito, et al.; Blood 117, 4968 (2011), Abstract;

NAADP mediates ATP-induced Ca(2+) signals in astrocytes: M. Barcelo-Torns, et al.; FEBS Lett. 585, 2300 (2011), Abstract;

Nicotinic acid adenine dinucleotide phosphate (NAADP) regulates autophagy in cultured astrocytes: G.J. Pereira, et al.; J. Biol. Chem. in press, (2011), Abstract; Full Text

Reconstitution of lysosomal NAADP-TRP-ML1 signaling pathway and its function in TRP-ML1(-/-) cells: F. Zhang, et al.; Am. J. Physiol. Cell Physiol. 301, C421 (2011), Abstract;

The endo-lysosomal system as an NAADP-sensitive acidic Ca(2+) store: role for the two-pore channels: S. Patel, et al.; Cell Calcium 50, 157 (2011), Abstract;

A functional role for nicotinic acid adenine dinucleotide phosphate in oxytocin-mediated contraction of uterine smooth muscle from rat: P.K. Aley, et al.; J. Pharmacol. Exp. Ther. 333, 726 (2010), Abstract;

Acidic NAADP-sensitive calcium stores in the endothelium: agonist-specific recruitment and role in regulating blood pressure: G.C. Brailoiu, et al.; J. Biol. Chem. 285, 37133 (2010), Abstract;

Acute insulin signaling in pancreatic beta-cells is mediated by multiple Raf-1 dependent pathways: E.U. Alejandro, et al.; Endocrinol. 151, 502 (2010), Abstract;

Deviant nicotinic acid adenine dinucleotide phosphate (NAADP)-mediated Ca2+ signaling upon lysosome proliferation: G.D. Dickinson, et al.; J. Biol. Chem. 285, 13321 (2010), Abstract;

Expecting to eat: glucagon-like peptide-1 and the anticipation of meals: D.L. Williams, et al.; Endocrinology 151, 445 (2010), Abstract; Full Text

Nicotinic acid adenine dinucleotide phosphate regulates skeletal muscle differentiation via action at two-pore channels: P.K. Aley, et al.; PNAS 107, 19927 (2010), Abstract;

Purified TPC Isoforms Form NAADP Receptors with Distinct Roles for Ca(2+) Signaling and Endolysosomal Trafficking: M. Ruas, et al.; Curr. Biol. 20, 703 (2010), Abstract;

TPC2 Is a Novel NAADP-sensitive Ca2+ Release Channel, Operating as a Dual Sensor of Luminal pH and Ca2+: S.J. Pitt, et al.; J. Biol. Chem. 285, 35039 (2010), Abstract;

Analogues of the nicotinic acid adenine dinucleotide phosphate (NAADP) antagonist Ned-19 indicate two binding sites on the NAADP receptor: D. Rosen, et al.; J. Biol. Chem. 284, 34930 (2009), Abstract;

Identification of a chemical probe for NAADP by virtual screening: E. Naylor, et al.; Nat. Chem. Biol. 4, 220 (2009), Abstract;

NAADP receptors mediate calcium signaling stimulated by endothelin-1 and norepinephrine in renal afferent arterioles: T.L. Thai, et al.; Am. J. Physiol. Renal. Physiol. 297, F510 (2009), Abstract;

NAADP: a universal Ca2+ trigger: A.H. Guse & H.C. Lee; Sci Signal 1, re10 (2008), Abstract;

General Literature References

The calcium-mobilizing messenger nicotinic acid adenine dinucleotide phosphate participates in sperm activation by mediating the acrosome reaction: S.R. Vasudevan, et al.; J. Biol. Chem. 285, 18262 (2010), Abstract;