Cell permeable, selective and potent inhibitor of nicotinic acid adenine dinucleotide phosphate (NAADP) receptor in mammalian cells and sea urchin eggs. Blocks NAADP signaling. Inhibits Ca2+ release: trans-Ned-19 (IC50 = 6nM); cis-Ned-19 (IC50 = 800nM). [32P] NAADP binding: trans-Ned-19 (IC50 = 0.4nM); cis-Ned-19 (IC50 = 15µM).
Inhibits Ca2+ release from lysosomes. Noncompetitive antagonist. Fluorescent.
Product Details
| Alternative Name: | 1-(3-((4-(2-Fluorophenyl)piperazin-1-yl)methyl)-4-methoxyphenyl)-2,3,4,9-tetrahydro-1H-pyrido[3,4-b]indole-3-carboxylic acid |
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| Formula: | C30H31FN4O3 |
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| MW: | 514.6 |
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| Source: | Synthetic |
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| Purity: | ≥97% (HPLC) |
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| Identity: | Determined by 1H-NMR and MS. |
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| Appearance: | White to off-white powder. |
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| Solubility: | Soluble in DMSO or 100% ethanol. |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | -20°C |
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| Handling: | Protect from light. |
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| Scientific Background: | NAADP (nicotinic acid adenine dinucleotide phosphate) is an emerging Ca2+ releasing second messenger in several mammalian tissues including pancreatic, brain and cardiac tissue. |
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| Technical Info/Product Notes: | Fluorescence of Ned-19 is compatible with visualization of the NAADP receptor in intact cells via confocal microscopy using an ultraviolet Argon ion laser (excitation 351 and 365 nm). |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Axonal transport of late endosomes and amphisomes is selectively modulated by local Ca2+ efflux and disrupted by PSEN1 loss of function: P.P. Y. Lie, et al.; Sci. Adv.
8, eabj5716 (2022),
Abstract;
NKG7 Is a T-cell–Intrinsic Therapeutic Target for Improving Antitumor Cytotoxicity and Cancer Immunotherapy: T. Wen, et al.; Cancer Immunol. Res.
10, 162 (2022),
Application(s): Treatment of primary human CD8+ T cell culture,
Abstract;
Full Text
TPC2-mediated Ca2+ signaling is required for the establishment of synchronized activity in developing zebrafish primary motor neurons: J.J. Kelu, et al.; Dev. Biol.
438, 57 (2018),
Abstract;
Full Text
CD38-mediated Ca(2+) signaling contributes to glucagon-induced hepatic gluconeogenesis: S.Y. Rah, et al.; Sci. Rep.
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Application(s): Cell Culture,
Abstract;
Full Text
Leucine-rich repeat kinase 2 regulates autophagy through a calcium-dependent pathway involving NAADP: P. Gomez-Suaga, et al.; Hum. Mol. Genet.
21, 511 (2012),
Abstract;
Full Text
NAADP regulates human platelet function: C.H. Coxon, et al.; Biochem. J.
441, 435 (2012),
Abstract;
Full Text
Actions of the NAADP Antagonist Trans-Ned19 in Cardiac Ventricular Myocytes: L. Elson, et al.; Biophys. J. 100, 81a (2011),
NAADP links histamine H1 receptors to secretion of von Willebrand factor in human endothelial cells: B. Esposito, et al.; Blood
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Abstract;
NAADP mediates ATP-induced Ca(2+) signals in astrocytes: M. Barcelo-Torns, et al.; FEBS Lett.
585, 2300 (2011),
Abstract;
Nicotinic acid adenine dinucleotide phosphate (NAADP) regulates autophagy in cultured astrocytes: G.J. Pereira, et al.; J. Biol. Chem.
286, 27875 (2011),
Abstract;
Full Text
Reconstitution of lysosomal NAADP-TRP-ML1 signaling pathway and its function in TRP-ML1(-/-) cells: F. Zhang, et al.; Am. J. Physiol. Cell Physiol.
301, C421 (2011),
Abstract;
The endo-lysosomal system as an NAADP-sensitive acidic Ca(2+) store: role for the two-pore channels: S. Patel, et al.; Cell Calcium
50, 157 (2011),
Abstract;
A functional role for nicotinic acid adenine dinucleotide phosphate in oxytocin-mediated contraction of uterine smooth muscle from rat: P.K. Aley, et al.; J. Pharmacol. Exp. Ther.
333, 726 (2010),
Abstract;
Acidic NAADP-sensitive calcium stores in the endothelium: agonist-specific recruitment and role in regulating blood pressure: G.C. Brailoiu, et al.; J. Biol. Chem.
285, 37133 (2010),
Abstract;
Acute insulin signaling in pancreatic beta-cells is mediated by multiple Raf-1 dependent pathways: E.U. Alejandro, et al.; Endocrinol.
151, 502 (2010),
Abstract;
Deviant nicotinic acid adenine dinucleotide phosphate (NAADP)-mediated Ca2+ signaling upon lysosome proliferation: G.D. Dickinson, et al.; J. Biol. Chem.
285, 13321 (2010),
Abstract;
Expecting to eat: glucagon-like peptide-1 and the anticipation of meals: D.L. Williams, et al.; Endocrinology
151, 445 (2010),
Abstract;
Full Text
Nicotinic acid adenine dinucleotide phosphate regulates skeletal muscle differentiation via action at two-pore channels: P.K. Aley, et al.; PNAS
107, 19927 (2010),
Abstract;
Purified TPC Isoforms Form NAADP Receptors with Distinct Roles for Ca(2+) Signaling and Endolysosomal Trafficking: M. Ruas, et al.; Curr. Biol.
20, 703 (2010),
Abstract;
TPC2 Is a Novel NAADP-sensitive Ca2+ Release Channel, Operating as a Dual Sensor of Luminal pH and Ca2+: S.J. Pitt, et al.; J. Biol. Chem.
285, 35039 (2010),
Abstract;
Analogues of the nicotinic acid adenine dinucleotide phosphate (NAADP) antagonist Ned-19 indicate two binding sites on the NAADP receptor: D. Rosen, et al.; J. Biol. Chem.
284, 34930 (2009),
Abstract;
Identification of a chemical probe for NAADP by virtual screening: E. Naylor, et al.; Nat. Chem. Biol.
4, 220 (2009),
Abstract;
NAADP receptors mediate calcium signaling stimulated by endothelin-1 and norepinephrine in renal afferent arterioles: T.L. Thai, et al.; Am. J. Physiol. Renal Physiol.
297, F510 (2009),
Abstract;
NAADP: a universal Ca2+ trigger: A.H. Guse & H.C. Lee; Sci. Signal.
1, re10 (2008),
Abstract;
General Literature References
The calcium-mobilizing messenger nicotinic acid adenine dinucleotide phosphate participates in sperm activation by mediating the acrosome reaction: S.R. Vasudevan, et al.; J. Biol. Chem.
285, 18262 (2010),
Abstract;
