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IκB inhibitor
ALX-270-245-M005 5 mg 142.00 USD
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Among the aldehydes formed, 4-HNE is the major product of lipid peroxidation, and it has been suggested to play a major role in liver toxicity associated with lipid peroxidation. Prevents NF-κB activation and TNF expression by inhibiting IκB phosphorylation and subsequent proteolysis.

Product Specification

Alternative Name:4-HNE
Formulation:Liquid. Solution in n-hexane.
Purity:≥90% (HPLC) (precursor dimethylacetal)
Solubility:Soluble in DMSO (washed with nitrogen) or 100% ethanol.
Reconstitution:Reconstitution in aqueous solutions:
Further dilutions of the stock solution into aqueous buffers or isotonic saline should be made prior to performing biological experiments. Ensure that the residual amount of organic solvent is insignificant, since organic solvents may have physiological effects at low concentrations. If an organic solvent-free aqueous solution is needed, it can be prepared by evaporating the n-Hexane and directly dissolving it in an aqueous buffer. Aqueous solutions of HNE should be kept at neutral pH, since HNE undergoes polymerization in basic and acidic conditions. The solubility of HNE in PBS (pH 7.2) is at least 1 mg/ml. Store aqueous solutions of HNE on ice and use within 6 hours of preparation.
Shipping:Shipped on Dry Ice
Long Term Storage:-80°C
Use/Stability:Store solutions on ice and use within 6 hours of preparation.
Handling:Protect from light and oxygen. Keep under inert gas. Avoid freeze/thaw cycles.
Technical Info/Product Notes:(E)-4-Hydroxynonenal-dimethylacetal (Prod. No. ALX-270-375) can be used for making a fresh preparation of HNE.
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Product Literature References

Aqueous or lipid components of atherosclerotic lesion increase macrophage oxidation and lipid accumulation: N. Abu-Saleh, et al.; Life Sci. 154, 1 (2016), Application(s): Immunofluorescent staining, Abstract;
Relation between TLR4/NF-κB signaling pathway activation by 27-hydroxycholesterol and 4-hydroxynonenal, and atherosclerotic plaque instability: S. Gargiulo, et al.; Aging Cell 14, 569 (2015), Application(s): Cell Culture, Abstract; Full Text
Transient Receptor Potential Ankyrin 1 is Expressed by Inhibitory Motoneurons of the Mouse Intestine: D.P. Poole, et al.; Gastroenterology 141, 565 (2011), Abstract;
IkappaB kinase, a molecular target for inhibition by 4-hydroxy-2- nonenal: C. Ji, et al.; J. Biol. Chem. 276, 18223 (2001), Abstract; Full Text
Multidrug resistance protein MRP1 protects against the toxicity of the major lipid peroxidation product 4-hydroxynonenal: J. Renes, et al.; Biochem. J. 350, 555 (2000), Abstract;
The lipid peroxidation product 4-hydroxy-2,3-nonenal inhibits constitutive and inducible activity of nuclear factor kappa B in neurons: S. Camandola, et al.; Brain Res. Mol. Brain Res. 85, 53 (2000), Abstract;
4-Hydroxynonenal prevents NF-kappaB activation and tumor necrosis factor expression by inhibiting IkappaB phosphorylation and subsequent proteolysis: S. Page, et al.; J. Biol. Chem. 274, 11611 (1999), Abstract; Full Text
Oxidative alterations in Alzheimer's disease: W.R. Markesbery & J.M. Carney; Brain Pathol. 9, 133 (1999), Abstract;
The lipid peroxidation product 4-hydroxynonenal impairs glutamate and glucose transport and choline acetyltransferase activity in NSC-19 motor neuron cells: W.A. Pedersen, et al.; Exp. Neurol. 155, 1 (1999), Abstract;
The lipid peroxidation product 4-hydroxynonenal inhibits neurite outgrowth, disrupts neuronal microtubules, and modifies cellular tubulin: M.D. Neely, et al.; J. Neurochem. 72, 2323 (1999), Abstract;
4-hydroxynonenal, a product of lipid peroxidation, is increased in the brain in Alzheimer's disease: W.R. Markesbery and M.A. Lovell; Neurobiol. Aging 19, 33 (1998), Abstract;
Effects of 4-hydroxynonenal, a lipid peroxidation product, on dopamine transport and Na+/K+ ATPase in rat striatal synaptosomes: P. Morel, et al.; Neurochem. Int. 33, 531 (1998), Abstract;
Genotoxic lipid peroxidation products: their DNA damaging properties and role in formation of endogenous DNA adducts: P.C. Burcham; Mutagenesis 13, 287 (1998), Abstract;
Histochemical detection of 4-hydroxynonenal protein in Alzheimer amyloid: Y. Ando, et al.; J. Neurol. Sci. 156, 172 (1998), Abstract;
Metabolism of 4-hydroxynonenal, a cytotoxic lipid peroxidation product, in thymocytes as an effective secondary antioxidative defense mechanism: W.G. Siems, et al.; J. Biochem. 123, 534 (1998), Abstract;
Modification of ion homeostasis by lipid peroxidation: roles in neuronal degeneration and adaptive plasticity: M.P. Mattson; TINS 21, 53 (1998), Abstract;
Roles of lipid peroxidation in modulation of cellular signaling pathways, cell dysfunction, and death in the nervous system: J.N. Keller & M.P. Mattson; Rev. Neurosci. 9, 105 (1998), Abstract;
Quantitation of 4-hydroxynonenal protein adducts: K. Uchida & E.R. Stadtman; Meth. Enzymol. 233, 371 (1994), Abstract;
Chemistry and biochemistry of 4-hydroxynonenal, malonaldehyde and related aldehydes: H. Esterbauer, et al.; Free Radic. Biol. Med. 11, 81 (1991), Abstract;
Suggested mechanisms for the production of 4-hydroxy-2-nonenal from the autoxidation of polyunsaturated fatty acids: W.A. Pryor & N.A. Porter; Free Radic. Biol. Med. 8, 541 (1990), Abstract;

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