Phenylmethylsulfonyl fluoride

Inhibitor of serine proteases and FAAH



ALX-270-184-G001	1 g	26.00 USD

ALX-270-184-G005	5 g	55.00 USD

ALX-270-184-G025	25 g	147.00 USD

Irreversible inhibitor of serine proteases. Widely used fatty acid amid hydrolase (FAAH) inhibitor for pretreatment of brain membrane preparations when testing CB₁ receptor activity. Spleen membranes used for CB₂ studies do not require this pretreatment, as FAAH activity is absent in these cells. Inhibits also cysteine proteases like papain (reversible by DTT treatment, Prod. No. ALX-280-001), as well as internucleosomal DNA fragmentation in immature thymocytes. For a related, more stable inhibitor see AEBSF (Prod. No. ALX-270-022).

Product Specification

Alternative Name:	PMSF, Benzylsulfonyl fluoride

Formula:	C₇H₇FO₂S

MW:	174.2

Purity:	≥99% (HPLC)

Appearance:	White to off-white needle crystals.

CAS:	329-98-6

RTECS:	XT8040000

Solubility:	Soluble in 100% ethanol, methanol or isopropanol; insoluble in water.

Long Term Storage:	+20°C

Hazard:	CORROSIVE. HARMFUL.

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Product Literature References

Enzymatic synthesis and degradation of anandamide, a cannabinoid receptor agonist: D.G. Deutsch & S.A. Chin; Biochem. Pharmacol. 46, 791 (1993), Abstract;

Inactivation of the protease inhibitor phenylmethylsulfonyl fluoride in buffers: G.T. James; Anal. Biochem. 86, 574 (1978), Abstract;

Kinetic analysis of differences in brain acetylcholinesterase from fish or mammalian sources: D.E. Moss & D.E. Fahrney; Biochem. Pharmacol. 27, 2693 (1978), Abstract;