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Z-VAD-FMK (Ready-to-Use)

Caspase inhibitor
 
ALX-260-138-R100 100 µl 2mM 185.00 USD
 
ALX-260-138-R020 20 µl 10mM 185.00 USD
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Synthetic peptide that irreversibly inhibits ICE-family protease/caspase activity and blocks apoptosis. The inhibitor is designed as a methyl ester to facilitate cell permeability.

Product Specification

Alternative Name:Caspase family inhibitor (fluoromethylketone)
 
Sequence:Z-Val-Ala-Asp-fluoromethylketone
 
Formula:C22H30FN3O7
 
MW:467.5
 
Source:Synthetic
 
Formulation:Liquid. In DMSO.
 
Purity:≥99% (TLC)
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Technical Info/Product Notes:We recommend a final concentration of 2-10µM for inhibiting caspase activity in Jurkat cell culture. The optimal doses may vary for different cells and culture conditions.
 
260-138
Figure: Apoptosis was induced in Jurkat cells with camptothecin (2µM) for 6 hours in the presence of various concentrations of Z-VAD-FMK (Prod. No. ALX-260-138). Caspase activity was measured using Caspase-3 Fluorometric Assay Kit (Prod. No. ALX-850-216) according to the kit instructions.
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260-138

Product Literature References

Lysosomal Degradation of CD44 Mediates Ceramide Nanoliposome-induced Anoikis and Diminished Extravasation in Metastatic Carcinoma Cells: J. K. Haakenson, et al.; J. Biol. Chem. 290, 8632 (2015), Application(s): Cell Culture , Abstract; Full Text

General Literature References

Baculovirus p35 and Z-VAD-fmk inhibit thapsigargin-induced apoptosis of breast cancer cells: X.M. Qi, et al.; Oncogene 15, 1207 (1997), Abstract;
Benzyloxycarbonyl-Val-Ala-Asp (OMe) fluoromethylketone (Z-VAD.FMK) inhibits apoptosis by blocking the processing of CPP32: E.A. Slee, et al.; Biochem. J. 315, 21 (1996), Abstract; Full Text
Fas-induced activation of the cell death-related protease CPP32 Is inhibited by Bcl-2 and by ICE family protease inhibitors: R.C. Armstrong, et al.; J. Biol. Chem. 271, 16850 (1996), Abstract; Full Text

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