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Ac-VDVAD-AFC

Caspase-2 substrate
 
ALX-260-112-M005 5 mg 223.00 USD
 
ALX-260-112-M010 10 mg 398.00 USD
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Replaces Prod. #: BML-P464

Fluorogenic substrate for caspase-2; similar to Ac-VDVAD-AMC but the AFC fluorophore has a greater Stokes’ shift upon cleavage. VDVAD has been found to be a preferred cleavage site for caspase-2 (Ich-1L). Increases in Ac-VDVAD-AFC cleavage correlated with losses in procaspase-2 (conversion to active caspase-2) in human neuroblastoma lines induced to apoptosis with C2-ceramide and NO.

Product Specification

Alternative Name:Caspase-2 substrate (fluorogenic)
 
Sequence:Ac-Val-Asp-Val-Ala-Asp-AFC (AFC=7-Amino-4-trifluoromethylcoumarin)
 
Formula:C33H41F3N6O12
 
MW:770.7
 
Peptide Content:75-95%
 
Purity:≥98% (HPLC)
 
Appearance:White to off-white powder.
 
Solubility:Soluble in DMSO.
 
Shipping:Ambient
 
Long Term Storage:-20°C
 
Handling:Warm up to room temperature before opening. Keep cool and dry.
 
Technical Info/Product Notes:AFC has an excitation maximum of 400nm and an emission maximum of 505nm.
 

General Literature References

Mechanism of nitric oxide-induced apoptosis in human neuroblastoma SH-SY5Y cells: R. Moriya, et al.; FEBS Lett. 484, 253 (2000), Abstract;
Possible involvement of cytochrome c release and sequential activation of caspases in ceramide-induced apoptosis in SK-N-MC cells: A. Ito, et al.; Biochim. Biophys. Acta 1452, 263 (1999), Abstract;
Substrate specificities of caspase family proteases: R.V. Talanian, et al.; J. Biol. Chem. 272, 9677 (1997), Abstract;

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