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Ac-IETD-AFC

Caspase and granzyme substrate
 
ALX-260-110-M005 5 mg 175.00 USD
 
ALX-260-110-M010 10 mg 298.00 USD
Do you need bulk/larger quantities?
 
Replaces Prod. #: BML-P433

Fluorogenic substrate for caspase-8/caspase-3 processing enzyme and granzyme B. Also a substrate for caspase-10. The IETD sequence is based on caspase-3 proenzyme cleavage site.

Product Specification

Alternative Name:Caspase-8 substrate (fluorogenic), Granzyme B substrate (fluorogenic)
 
Sequence:Ac-Ile-Glu-Thr-Asp-AFC (AFC=7-Amino-4-trifluoromethylcoumarin)
 
Formula:C31H38F3N5O12
 
MW:729.7
 
Purity:≥98% (HPLC)
 
Appearance:White to off-white powder.
 
Solubility:Soluble in DMSO.
 
Shipping:Ambient
 
Long Term Storage:-20°C
 
Handling:Keep cool and dry.
 
Technical Info/Product Notes:AFC has an excitation maximum of 400nm and an emission maximum of 505nm.
 

Product Literature References

Artesunate induces ROS-dependent apoptosis via a Bax-mediated intrinsic pathway in Huh-7 and Hep3B cells: Y. Pang, et al.; Exp. Cell Res. 16, 30161 (2016), Application(s): Fluorometric assay for caspase-8 activity, Abstract;
Insights into the mechanism of human papillomavirus E2-induced procaspase-8 activation and cell death: N. Singh, et al.; Sci. Rep. 6, 21408 (2016), Application(s): Caspase-8 activity assay, Abstract;
Mechanism of neem limonoids-induced cell death in cancer: Role of oxidative phosphorylation: N. Yadav, et al.; Free Radic. Biol. Med. 90, 261 (2016), Application(s): Quantification of apoptosis and caspase activity measurement, Abstract;
Organ specific alteration in caspase expression and STK3 proteolysis during the aging process: M. Lessard-Beaudoin, et al.; Neurobiol. Aging 47, 50 (2016), Application(s): Caspase activity assays, Abstract; Full Text
Protolichesterinic acid enhances doxorubicin-induced apoptosis in HeLa cells in vitro: F. Brisdelli, et al.; Life Sci. 158, 89 (2016), Application(s): Caspase activity, Abstract;
A novel TNFR1-triggered apoptosis pathway mediated by class IA PI3Ks in neutrophils: B. Geering, et al.; Blood 117, 5953 (2011), Application(s): Caspase activity detected in human neutophils, Abstract; Full Text
Rapid cytochrome c release, activation of caspases 3, 6, 7 and 8 followed by Bap31 cleavage in HeLa cells treated with photodynamic therapy: D.J. Granville, et al.; FEBS Lett. 437, 5 (1998), Abstract;

General Literature References

Activation of a CrmA-insensitive, p35-sensitive pathway in ionizing radiation-induced apoptosis: R. Datta, et al.; J. Biol. Chem. 272, 1965 (1997), Abstract;
Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis: D.W. Nicholson, et al.; Nature 376, 37 (1995), Abstract;
Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE: Y.A. Lazebnik, et al.; Nature 371, 346 (1994), Abstract;
Interleukin-1 beta converting enzyme: N.A. Thornberry; Methods Enzymol. 244, 615 (1994), Abstract;

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