Ac-IETD-AFC

Caspase and granzyme substrate



ALX-260-110-M005	5 mg	145.00 USD

ALX-260-110-M010	10 mg	248.00 USD

Replaces Prod. #: BML-P433

Fluorogenic substrate for caspase-8/caspase-3 processing enzyme and granzyme B. Also a substrate for caspase-10. The IETD sequence is based on caspase-3 proenzyme cleavage site.

Product Specification

Alternative Name:	Caspase-8 substrate (fluorogenic), Granzyme B substrate (fluorogenic)

Formula:	C₃₁H₃₈F₃N₅O₁₂

MW:	729.7

Purity:	≥98% (HPLC)

Appearance:	White to off-white powder.

Sequence:	Ac-Ile-Glu-Thr-Asp-AFC (AFC=7-Amino-4-trifluoromethylcoumarin)

Solubility:	Soluble in DMSO.

Long Term Storage:	-20°C

Handling:	Keep cool and dry.

Background / Technical Information:	AFC has an excitation maximum of 400nm and an emission maximum of 505nm.

Product Literature References

Rapid cytochrome c release, activation of caspases 3, 6, 7 and 8 followed by Bap31 cleavage in HeLa cells treated with photodynamic therapy: D.J. Granville, et al.; FEBS Lett. 437, 5 (1998), Abstract;

General Literature References

Activation of a CrmA-insensitive, p35-sensitive pathway in ionizing radiation-induced apoptosis: R. Datta, et al.; J. Biol. Chem. 272, 1965 (1997), Abstract;

Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis: D.W. Nicholson, et al.; Nature 376, 37 (1995), Abstract;

Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE: Y.A. Lazebnik, et al.; Nature 371, 346 (1994), Abstract;

Interleukin-1 beta converting enzyme: N.A. Thornberry; Methods Enzymol. 244, 615 (1994), Abstract;