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Ac-WEHD-pNA

Substrate for caspase-1, -4, -5 and -14
 
ALX-260-082-M005 5 mg 128.00 USD
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Replaces Prod. #: BML-P451

Colorimetric caspase substrate. Combinatorial studies have found the WEHD sequence to be optimal for clevage of tetrapeptide substrate by caspase-1, caspase-4, caspase-5 and caspase-14. Cleavage of the substrate by a caspase releases pNA, which can be detected by its absorbance at 405nm.

Product Specification

Alternative Name:Caspase-1 substrate (chromogenic), Caspase-5 substrate (chromogenic)
 
Sequence:Ac-Trp-Glu-His-Asp-pNA (pNA=p-Nitroaniline)
 
Formula:C34H37N9O11
 
MW:747.7
 
Formulation:Lyophilized.
 
Peptide Content:70-90%
 
Purity:≥98% (HPLC)
 
Appearance:Yellow powder.
 
Solubility:Soluble in DMSO. Dilute with buffer, pH 7.5.
 
Shipping:Ambient
 
Long Term Storage:-20°C
 
Handling:Keep cool and dry.
 
Technical Info/Product Notes:λmax of pNA is 400nm.
 

Product Literature References

Purification and catalytic properties of human caspase family members: M. Garcia-Calvo et al.; Cell Death Differ. 6, 362 (1999), Abstract;
A combinatorial approach defines specificities of members of the caspase family and granzyme B. Functional relationships established for key mediators of apoptosis: N.A. Thornberry, et al.; J. Biol. Chem. 272, 17907 (1997), Abstract; Full Text

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For Research Use Only. Not for use in diagnostic procedures.
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