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Z-YVAD(OMe)-FMK

Inhibitor of caspase-1 and -4
 
ALX-260-074-M001 1 mg 380.00 USD
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Irreversible, cell permeable inhibitor of caspase-1. Inhibits also caspase-4.

Product Specification

Alternative Name:Caspase-1 inhibitor (fluoromethylketone)
 
Sequence:Z-Tyr-Val-Ala-Asp(OMe)-fluoromethylketone
 
Formula:C31H39FN4O9
 
MW:630.7
 
Purity:≥98% (TLC)
 
Appearance:White to off-white powder.
 
Solubility:Soluble in DMSO.
 
Shipping:Ambient
 
Long Term Storage:-20°C
 
Use/Stability:Stock solutions should be divided into aliquots and stored at -20°C.
 
Handling:Packaged under inert gas.
 
Technical Info/Product Notes:The carboxyl groups are OMe ester forms to enhance cell permeability. If purified enzyme is used, esterase should be added for the hydrolysis of the ester groups.
 
Protocol:Prepare a 10mM stock solution of the caspase inhibitor in high quality DMSO. Add 2µl of this stock solution to 1 ml of culture medium containing cells to give 20µM final concentration. Effective final concentrations are estimated to be 5-20µM. If a particular caspase inhibitor is applied at higher concentrations specificity for an individual caspase or even for the caspase family (>100µM) might be compromised. DMSO concentration above 0.2% may cause cellular toxicity thus masking the effect of the caspase inhibitor. For in vivo experiments extending for 12 to 48 hours, fresh inhibitor may have to be added (injected) due to inactivation by reaction with cysteine protease.
 

Product Literature References

Increased expression of the NLRP3 inflammasome components in patients with Behçet's disease: E.H. Kim, et al.; J. Inflamm. (Lond) 12, 41 (2015), Application(s): Cell Culture, qRT-PCR, Abstract; Full Text
Protective effect of a caspase inhibitor in models for cerebral ischemia in vitro and in vivo: C. Wiessner, et al.; Cell Mol. Biol. (Noisy-Le-Grand) 46, 53 (2000), Abstract;
Inhibition of ubiquitin-proteasome pathway activates a caspase-3-like protease and induces Bcl-2 cleavage in human M-07e leukaemic cells: X.M. Zhang, et al.; Biochem. J. 340, 127 (1999), Abstract; Full Text
The role of CED-3-related cysteine proteases in apoptosis of cerebellar granule cells: B.A. Eldadah, et al.; J. Neurosci. 17, 6105 (1997), Abstract; Full Text

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