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Ac-VDVAD-AMC

Caspase-2 substrate
 
ALX-260-060-M001 1 mg 51.00 USD
 
ALX-260-060-M005 5 mg 201.00 USD
Do you need bulk/larger quantities?
 
Replaces Prod. #: BML-P462

Fluorogenic substrate for caspase-2. Preferred cleavage site for caspase-2 (Km=80μM).

Product Specification

Alternative Name:Caspase-2 substrate (fluorogenic)
 
Sequence:Ac-Val-Asp-Val-Ala-Asp-AMC (AMC=7-Amino-4-methylcoumarin)
 
Formula:C33H44N6O12
 
MW:716.8
 
Peptide Content:75-95%
 
Purity:≥98% (HPLC)
 
Appearance:White to off-white powder.
 
Solubility:Soluble in DMSO (100mM). Dilute with distilled water or buffer, pH 7.0-7.5 (e. g. TRIS, HEPES).
 
Shipping:Ambient
 
Long Term Storage:-20°C
 
Handling:Keep cool and dry.
 
Technical Info/Product Notes:AMC has an excitation maximum of 340-360nm and an emission maximum of 440-460nm.
 

Product Literature References

Effect of mitochondrial fission inhibition on C2C12 differentiation: D. Bloemberg, et al.; Data Brief 7, 634 (2016), Application(s): Caspase activity, Abstract; Full Text
CD 95-independent mechanisms of IL-2 deprivation-induced apoptosis in activated human lymphocytes: T. Hieronymus, et al.; Cell Death Differ. 7, 538 (2000), Abstract;
Substrate specificities of caspase family proteases: R.V. Talanian, et al.; J. Biol. Chem. 272, 9677 (1997), Abstract; Full Text

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