Replaces Prod. #: BML-P431
Chromogenic substrate for caspase-6, -8 and granzyme B. Also a substrate for caspase-10. Similar to Ac-IETD-AMC and Ac-IETD-AFC but cleavage is monitored colorimetrically by absorbance at 405 nm.
Product Details
| Alternative Name: | Caspase-8 substrate (chromogenic), Granzyme B substrate (chromogenic) |
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| Sequence: | Ac-Ile-Glu-Thr-Asp-pNA (pNA=p-Nitroaniline) |
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| Formula: | C27H38N6O12 |
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| MW: | 638.6 |
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| Formulation: | Lyophilized. |
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| Peptide Content: | 70-90% |
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| Purity: | ≥95% (HPLC) |
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| Appearance: | White to yellow powder. |
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| Solubility: | Soluble in DMSO. Dilute with buffer, pH 7.0-7.5. |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | -20°C |
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| Handling: | Keep cool and dry. |
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| Technical Info/Product Notes: | λmax of pNA is 400nm. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Cardioprotective effects of Prolame and SNAP are related with nitric oxide production and with diminution of caspases and calpain-1 activities in reperfused rat hearts: N.G. Roman-Anguiano, et al.; PeerJ
7, e7348 (2019),
Abstract;
Full Text
Apoptosome-independent pathway for apoptosis. Biochemical analysis of APAF-1 defects and biological outcomes: C.A. Belmokhtar, et al.; J. Biol. Chem.
278, 29571 (2003),
Abstract;
Full Text
Apicidin, a histone deacetylase inhibitor, induces apoptosis and Fas/Fas ligand expression in human acute promyelocytic leukemia cells: S.H. Kwon, et al.; J. Biol. Chem.
277, 2073 (2002),
Abstract;
Full Text
A sequential two-step mechanism for the production of the mature p17:p12 form of caspase-3 in vitro: Z. Han, et al.; J. Biol. Chem.
272, 13432 (1997),
Abstract;
Full Text
Activation of a CrmA-insensitive, p35-sensitive pathway in ionizing radiation-induced apoptosis: R. Datta, et al.; J. Biol. Chem.
272, 1965 (1997),
Abstract;
Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis: D.W. Nicholson, et al.; Nature
376, 37 (1995),
Abstract;
Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE: Y.A. Lazebnik, et al.; Nature
371, 346 (1994),
Abstract;
Interleukin-1 beta converting enzyme: N.A. Thornberry; Methods Enzymol.
244, 615 (1994),
Abstract;
