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Ac-IETD-pNA

Caspase and granzyme substrate
 
ALX-260-045-M001 1 mg 97.00 USD
 
ALX-260-045-M005 5 mg 115.00 USD
Do you need bulk/larger quantities?
 
Replaces Prod. #: BML-P431

Chromogenic substrate for caspase-6, -8 and granzyme B. Also a substrate for caspase-10. Similar to Ac-IETD-AMC and Ac-IETD-AFC but cleavage is monitored colorimetrically by absorbance at 405 nm.

Product Specification

Alternative Name:Caspase-8 substrate (chromogenic), Granzyme B substrate (chromogenic)
 
Sequence:Ac-Ile-Glu-Thr-Asp-pNA (pNA=p-Nitroaniline)
 
Formula:C27H38N6O12
 
MW:638.6
 
Formulation:Lyophilized.
 
Peptide Content:70-90%
 
Purity:≥98% (HPLC)
 
Appearance:White to yellow powder.
 
Solubility:Soluble in DMSO. Dilute with buffer, pH 7.0-7.5.
 
Shipping:Ambient
 
Long Term Storage:-20°C
 
Handling:Keep cool and dry.
 
Technical Info/Product Notes:λmax of pNA is 400nm.
 

Product Literature References

Apoptosome-independent pathway for apoptosis. Biochemical analysis of APAF-1 defects and biological outcomes: C.A. Belmokhtar, et al.; J. Biol. Chem. 278, 29571 (2003), Abstract; Full Text
Apicidin, a histone deacetylase inhibitor, induces apoptosis and Fas/Fas ligand expression in human acute promyelocytic leukemia cells: S.H. Kwon, et al.; J. Biol. Chem. 277, 2073 (2002), Abstract; Full Text
A sequential two-step mechanism for the production of the mature p17:p12 form of caspase-3 in vitro: Z. Han, et al.; J. Biol. Chem. 272, 13432 (1997), Abstract; Full Text
Activation of a CrmA-insensitive, p35-sensitive pathway in ionizing radiation-induced apoptosis: R. Datta, et al.; J. Biol. Chem. 272, 1965 (1997), Abstract;
Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis: D.W. Nicholson, et al.; Nature 376, 37 (1995), Abstract;
Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE: Y.A. Lazebnik, et al.; Nature 371, 346 (1994), Abstract;
Interleukin-1 beta converting enzyme: N.A. Thornberry; Methods Enzymol. 244, 615 (1994), Abstract;

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For Research Use Only. Not for use in diagnostic procedures.
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