Ac-IETD-pNA

Caspase and granzyme substrate



ALX-260-045-M001	1 mg	26.00 USD

ALX-260-045-M005	5 mg	96.00 USD

Replaces Prod. #: BML-P431

Chromogenic substrate for caspase-6, -8 and granzyme B. Also a substrate for caspase-10. Similar to Ac-IETD-AMC and Ac-IETD-AFC but cleavage is monitored colorimetrically by absorbance at 405 nm.

Product Specification

Alternative Name:	Caspase-8 substrate (chromogenic), Granzyme B substrate (chromogenic)

Formula:	C₂₇H₃₈N₆O₁₂

MW:	638.6

Purity:	98% (HPLC)

Appearance:	White to yellow powder.

Sequence:	Ac-Ile-Glu-Thr-Asp-pNA (pNA=p-Nitroaniline)

Formulation:	Lyophilized.

Peptide Content:	70-90%

Solubility:	Soluble in DMSO. Dilute with buffer, pH 7.0-7.5.

Long Term Storage:	-20°C

Handling:	Keep cool and dry.

Background / Technical Information:	λmax of pNA is 400nm.

Product Literature References

Apoptosome-independent pathway for apoptosis. Biochemical analysis of APAF-1 defects and biological outcomes: C.A. Belmokhtar, et al.; J. Biol. Chem. 278, 29571 (2003), Abstract; Full Text

Apicidin, a histone deacetylase inhibitor, induces apoptosis and Fas/Fas ligand expression in human acute promyelocytic leukemia cells: S.H. Kwon, et al.; J. Biol. Chem. 277, 2073 (2002), Abstract; Full Text

A sequential two-step mechanism for the production of the mature p17:p12 form of caspase-3 in vitro: Z. Han, et al.; J. Biol. Chem. 272, 13432 (1997), Abstract; Full Text

Activation of a CrmA-insensitive, p35-sensitive pathway in ionizing radiation-induced apoptosis: R. Datta, et al.; J. Biol. Chem. 272, 1965 (1997), Abstract;

Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis: D.W. Nicholson, et al.; Nature 376, 37 (1995), Abstract;

Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE: Y.A. Lazebnik, et al.; Nature 371, 346 (1994), Abstract;

Interleukin-1 beta converting enzyme: N.A. Thornberry; Methods Enzymol. 244, 615 (1994), Abstract;