Ac-IETD-AMC

Caspase and granzyme substrate



ALX-260-042-M001	1 mg	40.00 USD

ALX-260-042-M005	5 mg	124.00 USD

Replaces Prod. #: BML-P432

Fluorogenic substrate for caspase-6 and -8 and granzyme B. Also a substrate for caspase-10.

Product Specification

Alternative Name:	Granzyme B substrate (fluorogenic), Caspase-8 substrate (fluorogenic)

Formula:	C₃₁H₄₁N₅O₁₂

MW:	675.7

Purity:	≥98% (HPLC)

Appearance:	White to off-white powder.

Sequence:	Ac-Ile-Glu-Thr-Asp-AMC (AMC=7-Amino-4-methylcoumarin)

Peptide Content:	70-90%

Solubility:	Soluble in DMSO. Dilute with buffer, pH 7.5.

Long Term Storage:	-20°C

Handling:	Keep cool and dry. Protect from light.

Background / Technical Information:	AMC has an excitation maximum of 340-360nm and an emission maximum of 440-460nm.

Product Literature References

Bid truncation, bid/bax targeting to the mitochondria, and caspase activation associated with neutrophil apoptosis are inhibited by granulocyte colony-stimulating factor: N.A. Maianski, et al.; J. Immunol. 172, 7024 (2004), Abstract;

Activation of a CrmA-insensitive, p35-sensitive pathway in ionizing radiation-induced apoptosis: R. Datta, et al.; J. Biol. Chem. 272, 1965 (1997), Abstract;

Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis: D.W. Nicholson, et al.; Nature 376, 37 (1995), Abstract;

Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE: Y.A. Lazebnik, et al.; Nature 371, 346 (1994), Abstract;

Interleukin-1 beta converting enzyme: N.A. Thornberry; Methods Enzymol. 244, 615 (1994), Abstract;