Replaces Prod. #: BML-P432
Fluorogenic substrate for caspase-6 and -8 and granzyme B. Also a substrate for caspase-10.
Product Details
| Alternative Name: | Granzyme B substrate (fluorogenic), Caspase-8 substrate (fluorogenic) |
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| Sequence: | Ac-Ile-Glu-Thr-Asp-AMC (AMC=7-Amino-4-methylcoumarin) |
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| Formula: | C31H41N5O12 |
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| MW: | 675.7 |
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| Peptide Content: | 70-90% |
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| Purity: | ≥97% (HPLC) |
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| Appearance: | White to off-white powder. |
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| Solubility: | Soluble in DMSO. Dilute with buffer, pH 7.5. |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | -20°C |
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| Handling: | Protect from light. Keep cool and dry. |
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| Technical Info/Product Notes: | AMC has an excitation maximum of 340-360nm and an emission maximum of 440-460nm. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Involvement of Bid in the crosstalk between ferroptotic agent-induced ER stress and TRAIL-induced apoptosis: J.H. Kim, et al.; J. Cell. Physiol.
237, 4180 (2022),
Abstract;
C33-A cells transfected with E6*I or E6*II the short forms of HPV-16 E6, displayed opposite effects on cisplatin-induced apoptosis: C.E. Vaisman, et al.; Virus Res.
247, 94 (2018),
Abstract;
Caspase-1 Engagement and TLR-Induced c-FLIP Expression Suppress ASC/Caspase-8-Dependent Apoptosis by Inflammasome Sensors NLRP1b and NLRC8: N. Van Opdenbosch, et al.; Cell Rep.
21, 3427 (2017),
Abstract;
Full Text
The effect of ARC ablation on skeletal muscle morphology, function, and apoptotic signaling during aging: K. Vorobej, et al.; Exp. Gerontol.
101, 69 (2017),
Abstract;
Ruthenium Complexes Induce HepG2 Human Hepatocellular Carcinoma Cell Apoptosis and Inhibit Cell Migration and Invasion through Regulation of the Nrf2 Pathway: Y. Lu, et al.; Int. J. Mol. Sci.
17, E775 (2016),
Application(s): Caspase activation by Ru complexes,
Abstract;
Full Text
Treatment of Mycobacterium tuberculosis-Infected Macrophages with Poly(Lactic-Co-Glycolic Acid) Microparticles Drives NFκB and Autophagy Dependent Bacillary Killing: C. Lawlor, et al.; PLoS One
11, e0149167 (2016),
Application(s): Caspase activity assays,
Abstract;
Full Text
Bid truncation, bid/bax targeting to the mitochondria, and caspase activation associated with neutrophil apoptosis are inhibited by granulocyte colony-stimulating factor: N.A. Maianski, et al.; J. Immunol.
172, 7024 (2004),
Abstract;
Activation of a CrmA-insensitive, p35-sensitive pathway in ionizing radiation-induced apoptosis: R. Datta, et al.; J. Biol. Chem.
272, 1965 (1997),
Abstract;
Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis: D.W. Nicholson, et al.; Nature
376, 37 (1995),
Abstract;
Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE: Y.A. Lazebnik, et al.; Nature
371, 346 (1994),
Abstract;
Interleukin-1 beta converting enzyme: N.A. Thornberry; Methods Enzymol.
244, 615 (1994),
Abstract;
