Furin convertase substrate (fluorogenic)

ALX-260-040

Revised 08-Mar-10

Furin convertase substrate (fluorogenic)
SYNONYMS	Boc-RVRR-AMC Boc RVRR AMC
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	Convertases / Related Products / Furin

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-260-040-M001		1 mg	25.00 USD
ALX-260-040-M005		5 mg	90.00 USD
Replaces Prod. #: BML-P143

Product Specification

SEQUENCE:	Boc-Arg-Val-Arg-Arg-AMC (AMC=7-Amino-4-methylcoumarin)
FORMULA:	C₃₈H₆₂N₁₄O₈
MW:	843.0
CAS NUMBER:	136132-77-9
PURITY:	≥95% (HPLC)
APPEARANCE:	White to off-white powder.
SOLUBILITY:	Soluble in water (10mg/ml) or DMSO.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
HANDLING:	Protect from light.

Product Description

Fluorogenic substrate for furin/SPC1/PACE/PCSK3 and other subtilisin/kexin-like proprotein convertases such as Kex2, SPC2/PC2/PCSK2, SPC3/PCSK1, PACE4/SPC4/PCSK6, SPC5/PC4/PCSK4, SPC6/PC5/PC6/PCSK5, and SPC7/LPC/PC7/PC8/PCSK7. Can be used on live cells. Ex: 350 nm, Em: 460 nm, although the following Ex/Em can also be used: 330-380/440-490.

Product Specific Literature References

Purification and characterization of furin, a Kex2-like processing endoprotease, produced in Chinese hamster ovary cells: K. Hatsuzawa, et al.; J. Biol. Chem. 267, 16094 (1992) Abstract; Full Text
Human furin is a calcium-dependent serine endoprotease that recognizes the sequence Arg-X-X-Arg and efficiently cleaves anthrax toxin protective antigen: S.S. Molloy, et al.; J. Biol. Chem. 267, 16396 (1992) Abstract; Full Text
Purification and characteristics of the candidate prohormone processing proteases PC2 and PC1/3 from bovine adrenal medulla chromaffin granules: A.V. Azaryan, et al.; J. Biol. Chem. 270, 8201 (1995) Abstract
PACE4: a subtilisin-like endoprotease with unique properties: R.E. Mains, et al.; Biochem J. 321 (Pt 3), 587 (1997) Abstract
In vitro characterization of the novel proprotein convertase PC7: J.S. Munzer, et al.; J. Biol. Chem. 272, 19672 (1997) Abstract
Quantitative characterization of furin specificity. Energetics of substrate discrimination using an internally consistent set of hexapeptidyl methylcoumarinamides: D.J. Krysan, et al.; J. Biol. Chem. 274, 23229 (1999) Abstract
Enzymic characterization in vitro of recombinant proprotein convertase PC4: A. Basak, et al.; Biochem J. 343 (Pt 1), 29 (1999) Abstract
Molecular cloning of kpcA gene encoding a Kex2p-like endoprotease from Aspergillus nidulans: B.K. Kwon, et al.; Mol. Cells 12, 142 (2001) Abstract
Production, purification, and characterization of recombinant prohormone convertase 5 from baculovirus-infected insect cells: B.M. Cain, et al.; Protein Expr. Purif. 24, 227 (2002) Abstract