Replaces Prod. #: BML-P411
Fluorogenic substrate for caspase-3 with Km=9.7µM. Also a substrate for caspase-1, -4, -7 and -8. The peptide sequence is based on the PARP cleavage site Asp216 for caspase-3. Ex.: 340-360nm, Em.: 440-460nm
Product Details
| Alternative Name: | Caspase-3 substrate (fluorogenic) |
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| Sequence: | Ac-Asp-Glu-Val-Asp-AMC (AMC = 7-Amino-4-methylcoumarin) |
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| Formula: | C30H37N5O13 |
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| MW: | 675.7 |
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| Formulation: | Lyophilized. |
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| Purity: | ≥97% (HPLC) |
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| Solubility: | Soluble in DMSO; dilute with buffer, pH 7.5. |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | -20°C |
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| Handling: | Protect from light. |
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| Technical Info/Product Notes: | AMC has an excitation maximum of 340-360nm and an emission maximum of 440-460nm. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Bioluminescent RIPoptosome Assay for FADD/RIPK1 Interaction Based on Split Luciferase Assay in a Human Neuroblastoma Cell Line SH-SY5Y: P. Ghanavatian, et al.; Biosensors
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Characterization of new highly selective pyrazolo[4,3-d]pyrimidine inhibitor of CDK7: M. Kovalová, et al.; Biomed. Pharmacother.
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Cardioprotective Effects of Oroxylum indicum Extract Against Doxorubicin and Cyclophosphamide-Induced Cardiotoxicity: S.R. Pondugula, et al.; Cardiovasc. Toxicol.
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Exogenous Introduction of Initiator and Executioner Caspases Results in Different Apoptotic Outcomes: F. Anson, et al.; JACS Au
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β-hydroxybutyric acid attenuates oxidative stress and improves markers of mitochondrial function in the HT-22 hippocampal cell line: M. Majrashi, et al.; J. Integr. Neurosci.
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Early-onset impairment of the ubiquitin-proteasome system in dopaminergic neurons caused by α-synuclein: C. McKinnon, et al.; Acta Neuropathol. Commun.
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Full Text
Neuroprotective Effects of Methyl Caffeate against Hydrogen Peroxide-Induced Cell Damage: Involvement of Caspase 3 and Cathepsin D Inhibition: D. Jantas, et al.; Biomolecules
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Full Text
Vitamin B12b Enhances the Cytotoxicity of Diethyldithiocarbamate in a Synergistic Manner, Inducing the Paraptosis-Like Death of Human Larynx Carcinoma Cells: M. Solovieva, et al.; Biomolecules
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C33-A cells transfected with E6*I or E6*II the short forms of HPV-16 E6, displayed opposite effects on cisplatin-induced apoptosis: C.E. Vaisman, et al.; Virus Res.
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Loperamide, pimozide, and STF-62247 trigger autophagy-dependent cell death in glioblastoma cells: S. Zielke, et al.; Cell Death Dis.
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Caspase-6 Undergoes a Distinct Helix-Strand Interconversion upon Substrate Binding: K.B. Dagbay, et al.; J. Biol. Chem.
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Study of commonly used organophosphate pesticides that induced oxidative stress and apoptosis in peripheral blood lymphocytes of rats: A. Ojha, et al.; Hum. Exp. Toxicol.
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The effect of ARC ablation on skeletal muscle morphology, function, and apoptotic signaling during aging: K. Vorobej, et al.; Exp. Gerontol.
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Cross-talk between two antioxidants, thioredoxin reductase and heme oxygenase-1, and therapeutic implications for multiple myeloma: P.V. Raninga, et al.; Redox Biol.
8, 175 (2016),
Application(s): Caspase-3 activity assay,
Abstract;
Data on skeletal muscle apoptosis, autophagy, and morphology in mice treated with doxorubicin: T.L. Campbell, et al.; Data Brief
7, 786 (2016),
Application(s): Assessed caspase-3, -8, and -9 activity in mixed gastrocnemius homogenates,
Abstract;
Diclofenac induces proteasome and mitochondrial dysfunction in murine cardiomyocytes and hearts: R. Ghosh, et al.; Int. J. Cardiol.
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Application(s): Measurement of Caspase 3 activity - mouse heart, liver, and kidney,
Abstract;
Effect of mitochondrial fission inhibition on C2C12 differentiation: D. Bloemberg, et al.; Data Brief
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Application(s): Caspase activity,
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Efficient Activation of Apoptotic Signaling during Mitotic Arrest with AK301: A. Chopra, et al.; PLoS One.
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Application(s): Caspase-3 assay,
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Full Text
Enhanced apoptotic cancer cell killing after Foscan photodynamic therapy combined with fenretinide via de novo sphingolipid biosynthesis pathway: N.B. Boppana, et al.; J. Photochem. Photobiol. B
159, 191 (2016),
Application(s): DEVDase activity assay,
Abstract;
Increased apoptosis and browning of TAK1-deficient adipocytes protects against obesity: A. Sassmann-Schweda, et al.; JCI Insight
1, e81175 (2016),
Application(s): Caspase-3 activity assay,
Abstract;
Full Text
Induction of cell death by the novel proteasome inhibitor marizomib in glioblastoma in vitro and in vivo: C.A. Manton, et al.; Sci. Rep.
25, 6 (2016),
Application(s): Caspase 3/7 activity assay,
Abstract;
Full Text
Leukemia inhibitory factor (LIF) withdrawal activates mTOR signaling pathway in mouse embryonic stem cells through the MEK/ERK/TSC2 pathway: M.Y. Cherepkova, et al.; Cell Death Dis.
14, e2050 (2016),
Application(s): Measured caspase-3 activity in vitro,
Abstract;
Protolichesterinic acid enhances doxorubicin-induced apoptosis in HeLa cells in vitro: F. Brisdelli, et al.; Life Sci.
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Application(s): Caspase activity,
Abstract;
The de-ubiquitylating enzyme DUBA is essential for spermatogenesis in Drosophila: L. Koerver, et al.; Cell Death Differ.
23, 2019 (2016),
Application(s): DEVDase assay from testes lysate,
Abstract;
Treatment of Mycobacterium tuberculosis-Infected Macrophages with Poly(Lactic-Co-Glycolic Acid) Microparticles Drives NFκB and Autophagy Dependent Bacillary Killing: C. Lawlor, et al.; PLoS One
11, e0149167 (2016),
Application(s): Caspase activity assays,
Abstract;
Full Text
Accentuating Death Signaling in Colon Cancer Cells: A Chemical Biology Approach: A. Chopra; (2015), (PhD thesis),
Application(s): Caspase-3 assay,
Full Text
Assessment of sequential combination of 5-Fluorouracil-loaded-chitosan-nanoparticles and ALA-Photodynamic therapy on HeLa cell line: M. Benito-Miguel, et al.; Photodiagnosis Photodyn. Ther.
12, 466 (2015),
Application(s): Assay using HeLa cells,
Abstract;
Calpain-3 impairs cell proliferation and stimulates oxidative stress-mediated cell death in melanoma cells: D. Moretti, et al.; PLoS One
10, e0117258 (2015),
Application(s): Fluorometric Assay,
Abstract;
Full Text
Efficacy of panobinostat and marizomib in acute myeloid leukemia and bortezomib-resistant models: F.F. Corrales-Medina, et al.; Leuk. Res.
39, 371 (2015),
Application(s): Western Blotting,
Abstract;
Phospholamban overexpression in mice causes a centronuclear myopathy-like phenotype: V.A. Fajardo, et al.; Dis. Model Mech.
8, 999 (2015),
Application(s): Fluorescence,
Abstract;
Full Text
Differential apoptosis-related protein expression, mitochondrial properties, proteolytic enzyme activity, and DNA fragmentation between skeletal muscles: E.M. McMillan, et al.; Am. J. Physiol. Regul. Integr. Comp. Physiol.
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Full Text
Purification and catalytic properties of human caspase family members: M. Garcia-Calvo, et al.; Cell Death Differ.
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Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis: D.W. Nicholson, et al.; Nature
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