Ac-DEVD-AMC

Caspase substrate



ALX-260-031-M001	1 mg	42.00 USD

ALX-260-031-M005	5 mg	119.00 USD

Replaces Prod. #: BML-P411

Fluorogenic substrate for caspase-3 with K_m=9.7µM. Also a substrate for caspase-1, -4, -7 and -8. The peptide sequence is based on the PARP cleavage site Asp²¹⁶ for caspase-3. Ex.: 340-360nm, Em.: 440-460nm

Product Specification

Alternative Name:	Caspase-3 substrate (fluorogenic)

Formula:	C₃₀H₃₇N₅O₁₃

MW:	675.7

Purity:	≥97% (HPLC)

Sequence:	Ac-Asp-Glu-Val-Asp-AMC (AMC = 7-Amino-4-methylcoumarin)

Formulation:	Lyophilized.

Peptide Content:	70-95%

Solubility:	Soluble in DMSO; dilute with buffer, pH 7.5.

Long Term Storage:	-20°C

Handling:	Protect from light.

Background / Technical Information:	AMC has an excitation maximum of 340-360nm and an emission maximum of 440-460nm.

Product Literature References

Purification and catalytic properties of human caspase family members: M. Garcia-Calvo, et al.; Cell Death Differ. 6, 362 (1999), Abstract;

Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis: D.W. Nicholson, et al.; Nature 376, 37 (1995), Abstract;

Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE: Y.A. Lazebnik et al.; Nature 371, 346 (1994), Abstract;