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Ac-DEVD-AMC

Caspase substrate
 
ALX-260-031-M001 1 mg 57.00 USD
 
ALX-260-031-M005 5 mg 144.00 USD
Do you need bulk/larger quantities?
 
Replaces Prod. #: BML-P411

Fluorogenic substrate for caspase-3 with Km=9.7µM. Also a substrate for caspase-1, -4, -7 and -8. The peptide sequence is based on the PARP cleavage site Asp216 for caspase-3. Ex.: 340-360nm, Em.: 440-460nm

Product Specification

Alternative Name:Caspase-3 substrate (fluorogenic)
 
Sequence:Ac-Asp-Glu-Val-Asp-AMC (AMC = 7-Amino-4-methylcoumarin)
 
Formula:C30H37N5O13
 
MW:675.7
 
Formulation:Lyophilized.
 
Purity:≥97% (HPLC)
 
Solubility:Soluble in DMSO; dilute with buffer, pH 7.5.
 
Shipping:Ambient
 
Long Term Storage:-20°C
 
Handling:Protect from light.
 
Technical Info/Product Notes:AMC has an excitation maximum of 340-360nm and an emission maximum of 440-460nm.
 

Product Literature References

Cross-talk between two antioxidants, thioredoxin reductase and heme oxygenase-1, and therapeutic implications for multiple myeloma: P.V. Raninga, et al.; Redox Biol. 8, 175 (2016), Application(s): Caspase-3 activity assay, Abstract;
Data on skeletal muscle apoptosis, autophagy, and morphology in mice treated with doxorubicin: T.L. Campbell, et al.; Data Brief 7, 786 (2016), Application(s): Assessed caspase-3, -8, and -9 activity in mixed gastrocnemius homogenates, Abstract;
Diclofenac induces proteasome and mitochondrial dysfunction in murine cardiomyocytes and hearts: R. Ghosh, et al.; Int. J. Cardiol. 223, 923 (2016), Application(s): Measurement of Caspase 3 activity - mouse heart, liver, and kidney, Abstract;
Effect of mitochondrial fission inhibition on C2C12 differentiation: D. Bloemberg, et al.; Data Brief 7, 634 (2016), Application(s): Caspase activity, Abstract; Full Text
Efficient Activation of Apoptotic Signaling during Mitotic Arrest with AK301: A. Chopra, et al.; PLoS One. 11, e0153818 (2016), Application(s): Caspase-3 assay, Abstract; Full Text
Enhanced apoptotic cancer cell killing after Foscan photodynamic therapy combined with fenretinide via de novo sphingolipid biosynthesis pathway: N.B. Boppana, et al.; J. Photochem. Photobiol. B 159, 191 (2016), Application(s): DEVDase activity assay, Abstract;
Increased apoptosis and browning of TAK1-deficient adipocytes protects against obesity: A. Sassmann-Schweda, et al.; JCI Insight (2016), Application(s): Caspase-3 activity assay, Full Text
Induction of cell death by the novel proteasome inhibitor marizomib in glioblastoma in vitro and in vivo: C.A. Manton, et al.; Sci. Rep. 25, 6 (2016), Application(s): Caspase 3/7 activity assay, Abstract; Full Text
Leukemia inhibitory factor (LIF) withdrawal activates mTOR signaling pathway in mouse embryonic stem cells through the MEK/ERK/TSC2 pathway: M.Y. Cherepkova, et al.; Cell Death Dis. 14, e2050 (2016), Application(s): Measured caspase-3 activity in vitro, Abstract;
Protolichesterinic acid enhances doxorubicin-induced apoptosis in HeLa cells in vitro: F. Brisdelli, et al.; Life Sci. 158, 89 (2016), Application(s): Caspase activity, Abstract;
Study of commonly used organophosphate pesticides that induced oxidative stress and apoptosis in peripheral blood lymphocytes of rats: A. Ojha, et al.; Hum. Exp. Toxicol. (2016), Abstract;
The de-ubiquitylating enzyme DUBA is essential for spermatogenesis in Drosophila: L. Koerver, et al.; Cell Death Differ. (2016), Application(s): DEVDase assay from testes lysate, Abstract;
Treatment of Mycobacterium tuberculosis-Infected Macrophages with Poly(Lactic-Co-Glycolic Acid) Microparticles Drives NFκB and Autophagy Dependent Bacillary Killing: C. Lawlor, et al.; PLoS One 11, e0149167 (2016), Application(s): Caspase activity assays, Abstract; Full Text
Accentuating Death Signaling in Colon Cancer Cells: A Chemical Biology Approach: A. Chopra; (2015), (PhD thesis), Application(s): Caspase-3 assay, Full Text
Assessment of sequential combination of 5-Fluorouracil-loaded-chitosan-nanoparticles and ALA-Photodynamic therapy on HeLa cell line: M. Benito-Miguel, et al.; Photodiagnosis Photodyn. Ther. 12, 466 (2015), Application(s): Assay using HeLa cells, Abstract;
Calpain-3 impairs cell proliferation and stimulates oxidative stress-mediated cell death in melanoma cells: D. Moretti, et al.; PLoS One 10, e0117258 (2015), Application(s): Fluorometric Assay, Abstract; Full Text
Efficacy of panobinostat and marizomib in acute myeloid leukemia and bortezomib-resistant models: F.F. Corrales-Medina, et al.; Leuk. Res. 39, 371 (2015), Application(s): Western Blotting, Abstract;
Phospholamban overexpression in mice causes a centronuclear myopathy-like phenotype: V.A. Fajardo, et al.; Dis. Model Mech. 8, 999 (2015), Application(s): Fluorescence, Abstract; Full Text
Differential apoptosis-related protein expression, mitochondrial properties, proteolytic enzyme activity, and DNA fragmentation between skeletal muscles: E.M. McMillan, et al.; Am. J. Physiol. Regul. Integr. Comp. Physiol. 300, R531 (2011), Abstract; Full Text
Purification and catalytic properties of human caspase family members: M. Garcia-Calvo, et al.; Cell Death Differ. 6, 362 (1999), Abstract;
Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis: D.W. Nicholson, et al.; Nature 376, 37 (1995), Abstract;
Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE: Y.A. Lazebnik et al.; Nature 371, 346 (1994), Abstract;

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