Ac-DEVD-CHO

Caspase-3 inhibitor



ALX-260-030-M001	1 mg	50.00 USD

ALX-260-030-M005	5 mg	189.00 USD

Replaces Prod. #: BML-P410

Highly specific, potent and reversible inhibitor of caspase-3. K_i<1nM for purified caspase-3. IC₅₀=0.2nM for PARP cleavage in cultured human osteosarcoma cell extracts. In contrast, the caspase-1 (ICE) specific inhibitor YVAD-CHO has a K_i=12µM for caspase-3 and IC₅₀>10µM for PARP cleavage in osteosarcoma cell extracts. The DEVD sequence is based on PARP cleavage site Asp-216 for caspase-3.

Product Specification

Alternative Name:	Caspase-3 inhibitor (aldehyde), Caspase-7 inhibitor (aldehyde)

Formula:	C₂₀H₃₀N₄O₁₁

MW:	502.5

Purity:	≥95% (HPLC)

Sequence:	Ac-Asp-Glu-Val-Asp-CHO

Formulation:	Lyophilized.

CAS:	169332-60-9

Peptide Content:	70-90%.

Solubility:	Soluble in distilled water or DMSO.

Reconstitution:	Add 0.2ml DMSO per 1mg peptide. Shake well until all the contents are dissolved. This furnishes a 10mM solution of this material.

Long Term Storage:	-20°C

Use/Stability:	Stock solutions should be divided into aliquots and stored at -20°C. Solutions are stable for at least 1 month when stored at -20°C in the dark and protected from moisture.

Handling:	Keep cool and dry. Keep under nitrogen.

Product Literature References

Inhibition of ubiquitin-proteasome pathway activates a caspase-3-like protease and induces Bcl-2 cleavage in human M-07e leukaemic cells: X.M. Zhang, et al.; Biochem. J. 340, 127 (1999), Abstract; Full Text

Interferon gamma induces upregulation and activation of caspases 1, 3, and 8 to produce apoptosis in human erythroid progenitor cells: C. Dai & S.B. Krantz; Blood 93, 3309 (1999), Abstract;

Inhibition of human caspases by peptide-based and macromolecular inhibitors: M. Garcia-Calvo, et al.; J. Biol. Chem. 273, 32608 (1998), Abstract; Full Text

Inhibition of human caspases by peptide-based and macromolecular inhibitors: M. Garcia-Calvo et al.; J. Biol. Chem. 273, 32608 (1998), Abstract;

A sequential two-step mechanism for the production of the mature p17:p12 form of caspase-3 in vitro: Z. Han, et al.; J. Biol. Chem. 272, 13432 (1997), Abstract; Full Text

CPP32/apopain is a key interleukin 1 beta converting enzyme-like protease involved in Fas-mediated apoptosis: J. Schlegel, et al.; J. Biol. Chem. 271, 1841 (1996), Abstract; Full Text

ICE/CED3-like proteases as therapeutic targets for the control of inappropriate apoptosis: D.W. Nicholson; Nature Biotechnol. 14, 297 (1996), Abstract;

Sequential activation of ICE-like and CPP32-like proteases during Fas- mediated apoptosis: M. Enari, et al.; Nature 380, 723 (1996), Abstract;

Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis: D.W. Nicholson, et al.; Nature 376, 37 (1995), Abstract;

Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE: Y,A, Lazebnik et al.; Nature 371, 346 (1994), Abstract;