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Ac-YVAD-pNA

Caspase-1 substrate
 
ALX-260-026-M001 1 mg 41.00 USD
 
ALX-260-026-M005 5 mg 85.00 USD
Do you need bulk/larger quantities?
 
Replaces Prod. #: BML-P408

Sequence is a modification of pro-interleukin-1β Asp-116 cleavage site for caspase-1. Similar to Ac-YVAD-AMC but cleavage is monitored colorimetrically at 405nm.

Product Specification

Alternative Name:Caspase-1 substrate (chromogenic)
 
Sequence:Ac-Tyr-Val-Ala-Asp-pNA (pNA=p-Nitroaniline)
 
Formula:C29H36N6O10
 
MW:628.6
 
CAS:149231-66-3
 
Formulation:Lyophilized.
 
Peptide Content:75-95%
 
Purity:≥97% (HPLC)
 
Appearance:White to off-white powder.
 
Solubility:Soluble in DMSO. Dilute with buffer pH 7.5.
 
Shipping:Ambient
 
Long Term Storage:-20°C
 
Handling:Protect from light. Keep cool and dry.
 
Technical Info/Product Notes:λmax of pNA is 400nm.
 

Product Literature References

Interleukin-1 β converting enzyme: N.A. Thornberry; Meth. Enzymol. 244, 615 (1994), Abstract;
Peptidic p-nitroanilide substrates of interleukin-1 β-converting enzyme: L.A. Reiter; Int. J. Pept. Protein Res. 43, 87 (1994), Abstract;
A novel heterodimeric cysteine protease is required for interleukin-1 β processing in monocytes: N.A. Thornberry, et al.; Nature 356, 768 (1992), Abstract;

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