Caspase family inhibitor (fluoromethylketone), Z-Val-Ala-DL-Asp-FMK
Can be used as a broad spectrum caspase inhibitor in conjunction with apoptosis detection assays.
Soluble in DMSO or acetonitrile (10mg/ml).
Long Term Storage:
Keep sealed after removing from the freezer until its temperature equilibrates with room temperature. Dissolve only the amount needed at one time because of the possibility of decomposition in solution.
After reconstitution, prepare aliquots and store at -20°C.
Prepare a 10mM stock solution of the caspase inhibitor in high quality DMSO. Add 2µl of this stock solution to 1ml of culture medium containing cells to give 20µM final concentration. Effective final concentrations are estimated to be 5-20µM. If a particular caspase inhibitor is applied at higher concentrations specificity for an individual caspase or even for the caspase family (> 100µM) might be compromised. DMSO concentration above 0.2% may cause cellular toxicity thus masking the effect of the caspase inhibitor. For in vivo experiments extending for 12 to 48 hours, fresh inhibitor may have to be added (injected) due to inactivation by reaction with cysteine protease.
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Product Literature References
A trans-platinum(II) complex induces apoptosis in cancer stem cells of breast cancer: N. Aztopal, et al.; Bioorg. Med. Chem. 25, 269 (2017), Application(s): Cell treatment for the ATP viability assay, Abstract;
Innovative substance 2250 as a highly promising anti-neoplastic agent in malignant pancreatic carcinoma - in vitro and in vivo: M. Buchholz, et al.; BMC Cancer 17, 216 (2017), Abstract; Full Text
Ammonium accumulation is a primary effect of 2-methylcitrate exposure in an in vitro model for brain damage in methylmalonic aciduria: H.P. Cudré-Cung, et al.; Mol. Genet. Metab. 119, 57 (2016), Application(s): Cell culture, blocking apoptosis, Abstract;
Caspase-3 Deletion Promotes Necrosis in Atherosclerotic Plaques of ApoE Knockout Mice: M.O. Grootaert, et al.; Oxid. Med. Cell Longev. 2016, 3087 (2016), Application(s): Cell culture, mouse bone marrow-derived macrophages, Abstract; Full Text
Dasatinib promotes paclitaxel-induced necroptosis in lung adenocarcinoma with phosphorylated caspase-8 by c-Src: Y. Diao, et al.; Cancer Lett. 379, 12 (2016), Application(s): Cell culture, Abstract;
Increased apoptosis and browning of TAK1-deficient adipocytes protects against obesity: A. Sassmann-Schweda, et al.; JCI Insight (2016), Application(s): Blocked increased caspase-3 activity, Full Text
MASTL(Greatwall) regulates DNA damage responses by coordinating mitotic entry after checkpoint recovery and APC/C activation: P.Y. Wong, et al.; Sci. Rep. 6, 22230 (2016), Application(s): Cell culture, Abstract; Full Text
Mechanism of neem limonoids-induced cell death in cancer: Role of oxidative phosphorylation: N. Yadav, et al.; Free Radic. Biol. Med. 90, 261 (2016), Application(s): Quantification of apoptosis and caspase activity measurement, Abstract;
Organ specific alteration in caspase expression and STK3 proteolysis during the aging process: M. Lessard-Beaudoin, et al.; Neurobiol. Aging 47, 50 (2016), Application(s): Western blot analysis, Abstract; Full Text
PKC activation sensitizes basal-like breast cancer cell lines to Smac mimetics: L. Cornmark, et al.; Cell Death Discov. 2, Article number 16002 (2016), Application(s): Cell culture, Full Text
Pyrrocidine A, a metabolite of endophytic fungi, has a potent apoptosis-inducing activity against HL60 cells through caspase activation via the Michael addition: S. Uesugi, et al.; J. Antibiot. (Tokyo) 69, 113 (2016), Application(s): DNA gel electrophoresis, Abstract; Full Text
Ritonavir Interacts With Belinostat to Cause Endoplasmic Reticulum Stress and Histone Acetylation in Renal Cancer Cells: M. Isono, et al.; Oncol. Res. 24, 327 (2016), Application(s): Flow cytometry, Human renal cancer cells, Abstract; Full Text
Strong antitumor synergy between DNA crosslinking and HSP90 inhibition causes massive premitotic DNA fragmentation in ovarian cancer cells: D. Kramer, et al.; Cell Death Differ. (2016), Abstract;
The cellular apoptosis susceptibility protein (CAS) promotes TRAIL-induced apoptosis and cell proliferation: P. Monian & X. Jiang; J. Biol. Chem. 291, 2379 (2016), Application(s): Western blot, Abstract; Full Text
The de-ubiquitylating enzyme DUBA is essential for spermatogenesis in Drosophila: L. Koerver, et al.; Cell Death Differ. (2016), Application(s): DEVDase assay from testes lysate, Abstract;
Bile acid-induced necrosis in primary human hepatocytes and in patients with obstructive cholestasis: B. L. Woolbright, et al.; Toxicol. Appl. Pharmacol. 283, 168 (2015), Application(s): Cell Culture, Abstract;
Cellular responses to a prolonged delay in mitosis are determined by a DNA damage response controlled by Bcl-2 family proteins: D.J. Colin, et al.; Open Biol. 5, 140156 (2015), Application(s): Cell Culture, Abstract; Full Text
Characterization of Morphological and Cellular Events Underlying Oral Regeneration in the Sea Anemone, Nematostella vectensis: A.R. Amiel, et al.; Int. J. Mol. Sci. 16, 289449 (2015), Application(s): Blocked transcription, Abstract; Full Text
Cytochrome c oxidase deficiency accelerates mitochondrial apoptosis by activating ceramide synthase 6: S. Schüll, et al.; Cell Death Dis. 6, e1691 (2015), Application(s): Cell Culture, Abstract; Full Text
Differential roles of RIPK1 and RIPK3 in TNF-induced necroptosis and chemotherapeutic agent-induced cell death: K. Moriwaki, et al.; Cell Death Dis. 6, e1636 (2015), Application(s): Cell Culture, Abstract; Full Text
Fibril growth and seeding capacity play key roles in α-synuclein-mediated apoptotic cell death: A.L. Mahul-Mellier, et al.; Cell Death Differ. 22, 2107 (2015), Application(s): Cell Culture, Abstract;
Guggulsterone inhibits human cholangiocarcinoma Sk-ChA-1 and Mz-ChA-1 cell growth by inducing caspase-dependent apoptosis and downregulation of survivin and Bcl-2 expression: F. Zhong, et al.; Oncol. Lett. (2015), Application(s): Cell Culture, Full Text
Involvement of JNK and Caspase Activation in Hoiamide A-Induced Neurotoxicity in Neocortical Neurons: Z. Cao, et al.; Mar. Drugs 13, 903 (2015), Application(s): Cell Culture, Abstract; Full Text
The Tpl2 kinase regulates the COX-2/prostaglandin E2 axis in adipocytes in inflammatory conditions: F. Berthou, et al.; Mol. Endocrinol. 29, 1025 (2015), Application(s): Cell Culture, Abstract; Full Text
The Wnt target Peter Pan defines a novel p53-independent nucleolar stress response pathway: A.S. Pfister, et al.; J. Biol. Chem. 290, 10905 (2015), Application(s): Cell Culture, Abstract; Full Text
USP30 deubiquitylates mitochondrial Parkin substrates and restricts apoptotic cell death: J.R. Liang, et al.; EMBO Rep. 16, 618 (2015), Application(s): Cell Culture, Abstract; Full Text
TLR4 Activation Under Lipotoxic Conditions Leads to Synergistic Macrophage Cell Death Through a TRIF-Dependent Pathway: J. D. Schilling et al.; J. Immunol. 190, 1285 (2013), Application(s): Inhibition of caspase in macrophages, Abstract; Full Text
Cytotoxicity of troglitazone through PPARγ-independent pathway and p38 MAPK pathway in renal cell carcinoma: M. Fujita, et al.; Cancer Lett. 312, 219 (2011), Abstract;
Inhibition of poly(ADP-ribose) polymerase (PARP) induces apoptosis in lung cancer cell lines: N.N. Gangopadhyay, et al.; Cancer Invest. 29, 608 (2011), Abstract;
Type of cell death induced by various metal cations in cultured human gingival fibroblasts: R.G. Contreras, et al.; In Vivo 24, 513 (2010), Abstract;
Neuroprotection by a caspase inhibitor in acute bacterial meningitis: J.S. Braun, et al.; Nat. Med. 5, 298 (1999), Abstract;
An ICE-like protease is a common mediator of apoptosis induced by diverse stimuli in human monocytic THP.1 cells: H. Zhu, et al.; FEBS Lett. 374, 303 (1995), Abstract;