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MD-2 polyclonal antibody

 
ALX-215-063-C100 100 µg 300.00 USD
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Product Specification

Alternative Name:LY96, Lymphocyte antigen 96, ESOP-1
 
Host:Rabbit
 
Immunogen:Full length recombinant human MD-2.
 
UniProt ID:Q9Y6Y9
 
Species reactivity:Human, Mouse, Rat
 
Applications:IHC (PS), WB
 
Recommended Dilutions/Conditions:Immunohistochemistry (paraffin sections, 5-10µg/ml)
Western blot (0.5-2µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~30kDa by Western blot.
 
Purity Detail:Protein G-affinity purified
 
Formulation:Liquid. In PBS containing 0.2% gelatin and 0.05% sodium azide.
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Scientific Background:The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. Toll-like receptor 4 (TLR4), the principal signaling receptor for lipopolysaccharide (LPS) in mammals, requires the binding of MD-2 to its extracellular domain for maximal responsiveness. MD-2 enables TLR4 to respond to a wide variety of LPS partial structures, Gram-negative bacteria, and Gram-positive lipoteichoic acid, but not to Gram-positive bacteria, peptidoglycan, and lipopeptide.
 
MD-2 polyclonal antibody Western blot
Figure 1: Western blot analysis of MD-2 in human (A), mouse (B) and rat (C) spleen lysate using PAb to MD-2 (Prod. No. ALX-215-063) at 2μg/ml."
MD-2 polyclonal antibody Immunohistochemistry
Figure 2: Immunohistochemical staining of normal human brain using PAb to MD-2 (Prod. No. ALX-215-063) at 10μg/ml (A) or using a control rabbit Ig at 10μg/ml (B)."
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MD-2 polyclonal antibody Western blot MD-2 polyclonal antibody Immunohistochemistry

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