14-3-3η, pAb

[KCIP-1, Protein kinase C inhibitor protein 1, protein AS1]

Prod. # now: BML-SA476

Product Specification

Formulation:	Liquid. Neat serum. Contains no preservatives.

Immunogen:	Synthetic peptide corresponding to aa 1-11 (G¹DREQLLQRAR¹¹C) of 14-3-3η.

Source/Host:	From rabbit.

Specificity:	Recognizes human, mouse, rat, sheep, bovine and chicken 14-3-3η.

Application:	Immunohistochemistry (1:3200) Western Blot (1:3000) Optimal condition must be determined individually for each application.

Shipping:	SHIPPED ON BLUE ICE

Long Term Storage:	-20°C

Handling:	Avoid freeze/thaw cycles.

Product Literature References

Antibodies against the major brain isoforms of 14-3-3 protein. An antibody specific for the N-acetylated amino-terminus of a protein: H. Martin, et al.; FEBS Lett. 331, 296 (1993), Abstract; Full Text

Purification of 14-3-3 protein and analysis of isoforms in chicken brain: Y. Patel, et al.; Biochim. Biophys. Acta 1222, 405 (1994), Abstract;

Subcellular localisation of 14-3-3 isoforms in rat brain using specific antibodies: H. Martin, et al.; J. Neurochem. 63, 2259 (1994), Abstract;

Expression and structural analysis of 14-3-3 proteins: D.H. Jones, et al.; J. Mol. Biol. 245, 375 (1995), Abstract;

Isoforms of 14-3-3 protein can form homo- and heterodimers in vivo and in vitro: implications for function as adapter proteins: D.H. Jones, et al.; FEBS Lett. 368, 55 (1995), Abstract; Full Text

Identification of 14-3-3 proteins in human platelets: effects of synthetic peptides on protein kinase C activation: C.P. Wheeler-Jones, et al.; Biochem. J. 315, 41 (1996), Abstract; Full Text

14-3-3 inhibits the Dictyostelium myosin II heavy-chain-specific protein kinase C activity by a direct interaction: identification of the 14-3-3 binding domain: M. Matto-Yelin, et al.; Mol. Biol. Cell 8, 1889 (1997), Abstract; Full Text

Isoform pattern of 14-3-3 proteins in the cerebrospinal fluid of patients with Creutzfeldt-Jakob disease: J. Wiltfang, et al.; J. Neurochem. 73, 2485 (1999), Abstract;

Functional specificity in 14-3-3 isoform interactions through dimer formation and phosphorylation. Chromosome location of mammalian isoforms and variants: A. Aitken; Plant Mol. Biol. 50, 993 (2002), Abstract;

Immunolocalisation of 14-3-3 isoforms in normal and scrapie-infected murine brain: H.C. Baxter, et al.; Neuroscience 109, 5 (2002), Abstract;

Interaction of Akt-phosphorylated ataxin-1 with 14-3-3 mediates neurodegeneration in spinocerebellar ataxia type 1: H.K. Chen, et al.; Cell 113, 457 (2003), Abstract;

Unchanged survival rates of 14-3-3gamma knockout mice after inoculation with pathological prion protein: P. Steinacker, et al.; Mol. Cell. Biol. 25, 1339 (2005), Abstract; Full Text