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[pSer262]Tau polyclonal antibody

 
ALX-215-001-R100 100 µl 443.00 USD
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Product Specification

Alternative Name:Neurofibrillary tangle protein
 
Host:Rabbit
 
Immunogen:Synthetic peptide corresponding to the region of human tau containing phosphorylated Ser262.
 
UniProt ID:P10636
 
Species reactivity:Human
 
Crossreactivity:Expected to react with mouse, rat, goat, monkey, bovine and baboon tau.
 
Applications:WB
 
Recommended Dilutions/Conditions:Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Epitope affinity-purified. Negatively preadsorbed by non-phosphorylated immunogen peptide.
 
Formulation:Liquid. In Dulbecco’s PBS (without Mg2+ and Ca2+), pH 7.3, containing 50% glycerol, 1mg/ml BSA and 0.05% sodium azide.
 
Handling:For maximum product recovery after thawing, centrifuge the vial before opening the cap. Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Scientific Background:Tau phosphorylation at Ser202 and Ser262 is important for the regulation of microtubuli assembly.
 
Technical Info/Product Notes:Replacement for ADI-KAP-MA306
 
215-001

Figure: Peptide competition. Only the phosphopetide corresponding to tau [pS262] blocks the antibody signal, demonstrating the specificity of the antibody. Tau [pS262] phophorylation is induced by the addition of PKA.Method: Human recombinant tau untreated (lane 1) or treated with PKA (36μg per μg tau) for 45 min and added to background extracts was resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer overnight at 4°C, then incubated with PAb to Tau (phosphorylated) (pSer262) (Prod. No. ALX-215-001) for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the phosphopeptide immunogen (3), a generic phosphoserine-containing peptide (4), or the phosphopeptide immunogen (5). After washing, the membrane was incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and signals were detected using the Pierce SuperSignalTM method.

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215-001

Product Literature References

Dephosphorylation of tau by protein phosphatase 5: impairment in Alzheimer's disease: F. Liu, et al.; J. Biol. Chem. 280, (2005), Abstract; Full Text
Involvement of aberrant glycosylation in phosphorylation of tau by cdk5 and GSK-3beta: F. Liu, et al.; FEBS Lett. 530, 209 (2002), Abstract;
Role of glycosylation in hyperphosphorylation of tau in Alzheimer’s disease: F. Liu, et al.; FEBS Lett. 512, 101 (2002), Abstract;

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