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Goat anti-mouse IgG1 (human & mouse Ig adsorbed), (ATTO 590 conjugate)

 
ALX-211-204TM-C100 100 µg ATTO 590 144.00 USD
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A frequent cause of high background staining is due to non-specific binding of secondary antibodies. High quality secondary antibodies are crucial to obtain good staining of targets that are normally harder to detect, for example due to low expression levels. Interference caused by endogenous immunoglobulins can also result in poor detection. Secondary antibodies specifically adsorbed against cross-reacting immunoglobulins will enhance results.

Product Specification

Host:Goat
 
Immunogen:Mouse IgG1.
 
Species reactivity:Mouse
 
Specificity:Recognizes the heavy chain of mouse IgG1.
 
Crossreactivity:Minimally cross-reacts with human immunoglobulins.
 
Applications:ICC
 
Recommended Dilutions/Conditions:Immunocytochemistry (1:100)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Purified by affinity chromatography on mouse IgG1 covalently limited to agarose. Cross-adsorbed with mouse IgM, IgG2a, IgG2b, IgG3 and IgA, pooled human sera and purified human paraproteins.
 
Formulation:Liquid. In PBS, pH 7.4, containing 0.02% sodium azide.
 
Handling:Protect from light. Do not freeze.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:+4°C
 
Technical Info/Product Notes:ATTO-fluorescent antibodies show increased photostability, outstanding brightness and intense signals. ATTO dyes are thermally stable, resistant to environmental changes and show no significant isomerization. ATTO 590 shows bright red fluorescence (λabs (max): 594nm, λem (max): 624nm, εmax: 120’000).

Application Notes:
Image-Based Analysis of a Human Neurosphere Stem Cell Model for the Evaluation of Potential Neurotoxicants

Analysis of the Effect of Aggregated β-Amyloid on Cellular Signaling Pathways Critical for Memory in Alzheimer’s disease
 

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