Goat anti-rat IgM (rat Ig adsorbed), (HRP conjugate)



ALX-211-055-R050	50 µl	117.00 USD

A frequent cause of high background staining is due to non-specific binding of secondary antibodies. High quality secondary antibodies are crucial to obtain good staining of targets that are normally harder to detect, for example due to low expression levels. Interference caused by endogenous immunoglobulins can also result in poor detection. Secondary antibodies specifically adsorbed against cross-reacting immunoglobulins will enhance results.

Prod. No. ALX-211-055 is recommended for use with the following product:
ALX-804-131 MAb to BAFF (Buffy-2)

Product Specification

Purity Detail:	Purified by affinity chromatography on rat IgM covalently linked to Sepharose™-4B. Cross-adsorbed with rat IgG. Cross-reactivity with IgM from other species has not been evaluated.

Formulation:	Liquid. In PBS, pH 7.4, containing 50% glycerol. Contains no preservatives.

Immunogen:	Rat IgM.

Source/Host:	From goat.

Specificity:	Recognizes the heavy chain of rat IgM.

Application:	ELISA (1:4'000-1:8'000) Immunohistochemistry (frozen sections, paraffin sections) Western Blot (1:5'000) Optimal conditions must be determined individually for each application.

Short Term Storage:	+4°C

Long Term Storage:	-20°C

Handling:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Protect from light.

Background / Technical Information:	SEPHAROSE is a trademark of GE Healthcare companies.