Goat anti-rat IgG (mouse Ig adsorbed), (HRP conjugate)



ALX-211-052-R050	50 µl	186.00 USD

A frequent cause of high background staining is due to non-specific binding of secondary antibodies. High quality secondary antibodies are crucial to obtain good staining of targets that are normally harder to detect, for example due to low expression levels. Interference caused by endogenous immunoglobulins can also result in poor detection. Secondary antibodies specifically adsorbed against cross-reacting immuno-globulins will enhance results. Prod. No. ALX-211-052 has been adsorbed against cross-reacting mouse immunoglobulins for reduced background in mouse samples.

It is recommended for use with the following products:
ALX-804-010 MAb to FasL (H11)
ALX-804-125 MAb to FasL (Mike-2)
ALX-804-127 MAb to FLIP (Dave-2)
ALX-804-128 MAb to BAFF (Buffy-1)
ALX-804-202 MAb to E-Cadherin (ECCD-2)
ALX-804-258 MAb to α-Synuclein (15G7)
ALX-804-333 MAb to Smac/DIABLO (10G7)
ALX-804-366 MAb to Smac/DIABLO (9H10)

Product Specification

Purity Detail:	Purified by affinity chromatography on pooled rat IgG covalently linked to agarose. Cross-adsorbed with mouse sera and pooled mouse plasmacytoma or hybridoma proteins for minimal cross-reactivity with mouse IgG.

Formulation:	Liquid. In 50% glycerol/50% PBS, pH 7.4. Contains no preservatives.

Immunogen:	Rat IgG.

Source/Host:	From goat.

Specificity:	Recognizes the heavy and light chains of rat IgG, as well as the light chains of rat IgM and IgA.

Application:	ELISA (1:4'000-1:8'000) Immunohistochemistry (frozen sections, paraffin sections) Western Blot (1:5'000) Optimal conditions must be determined individually for each application.

Short Term Storage:	+4°C

Long Term Storage:	-20°C

Handling:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Protect from light.