Goat anti-rat IgG (mouse Ig adsorbed), (FITC conjugate)



ALX-211-051-C100	100 µg	130.00 USD

A frequent cause of high background staining is due to non-specific binding of secondary antibodies. High quality secondary antibodies are crucial to obtain good staining of targets that are normally harder to detect, for example due to low expression levels. Interference caused by endogenous immunoglobulins can also result in poor detection. Secondary antibodies specifically adsorbed against cross-reacting immuno-globulins will enhance results. Prod. No. ALX-211-051 has been adsorbed against cross-reacting mouse immunoglobulins for reduced background in mouse samples.

It is recommended for use with the following products:
ALX-804-010 MAb to FasL (H11)
ALX-804-127 MAb to FLIP (Dave-2)
ALX-804-190 MAb to Laminin-2α (4H8-2)
ALX-804-202 MAb to E-Cadherin (ECCD-2)
ALX-804-258 MAb to α-Synuclein (15G7)
ALX-804-333 MAb to Smac/DIABLO (10G7)
ALX-804-366 MAb to Smac/DIABLO (9H10)

Product Specification

Concentration:	1mg/ml.

Purity Detail:	Purified by affinity chromatography on pooled rat IgG covalently linked to agarose. Cross-adsorbed with mouse sera and pooled mouse plasmacytoma or hybridoma proteins for minimal cross-reactivity with mouse IgG.

Formulation:	Liquid. In PBS, pH 7.4, containing 0.1% sodium azide.

Immunogen:	Rat IgG.

Source/Host:	From goat.

Specificity:	Recognizes the heavy and light chains of rat IgG, as well as the light chains of rat IgM and IgA.

Application:	Flow Cytometry Immunocytochemistry Immunohistochemistry (frozen sections, paraffin sections) General recommended starting dilution is 1:100. Optimal conditions must be determined individually for each application.

Long Term Storage:	+4°C

Handling:	Do not freeze. Protect from light.

Crossreactivity Proteins:	IgG, IgM (light chains), IgA (light chains)