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PARP-3 polyclonal antibody

ALX-210-971-R100 100 µl 407.00 USD
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Product Specification

Alternative Name:Poly(ADP-ribose) polymerase-3
Immunogen:Recombinant human PARP-3 (aa 63-330).
UniProt ID:Q9Y6F1
Gene/Protein Identifier:10039 (Entrez Gene ID)
Species reactivity:Human, Mouse
Applications:ICC, IF, IHC (PS), WB
Recommended Dilutions/Conditions:Immunocytochemistry (1:100-1:500)
Immunofluorescence (1:100-1:500)
Immunohistochemistry (paraffin sections, 1:100-1:500)
Western Blot  (1:500-1:3000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Purity Detail:Affinity purified.
Formulation:Liquid. In 0.1M TRIS, pH 7.0, containing 0.1M glycine, 10% glycerol and 0.01% thimerosal (preservative).
Handling:Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:PARP-3 is part of the PARP family of enzymes that modify nuclear proteins by poly-ADP-ribosylation, which is required for DNA repair, regulation of apoptosis, and maintenance of genomic stability. This gene encodes the poly(ADP-ribosyl)transferase 3, which is preferentially localized to the daughter centriole throughout the cell cycle. Alternatively spliced transcript variants encoding different isoforms have been identified.
ALX-210-971 WB1
Western blot analysis in whole cell lysates of (target) in lanes A). A431 cells [30 µg] B). HeLa S3 cells [30 ug] using PARP-3, pAb (Prod. No. ALX-210-971) at a dilution of 1:1000.
ALX-210-971 WB2
Western blot analysis in mouse heart cell lysate [50 µg] of PARP3 using PARP-3, pAb (Prod. No. ALX-210-971) at a dilution of 1:1000.
ALX-210-971 IF
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells using PARP-3, pAb (Prod. No. ALX-210-971) at 1:500 dilution.
ALX-210-971 IHC
Immunohistochemical analysis of paraffin-embedded OVCA tissue using PARP-3, pAb (Prod. No. ALX-210-971) at 1:100 dilution.
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ALX-210-971 WB1 ALX-210-971 WB2 ALX-210-971 IF ALX-210-971 IHC

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The PARP Family & ADP Ribosylation
The PARP Family & ADP Ribosylation
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