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TIGAR polyclonal antibody (AT122)

 
ALX-210-947-C100 100 µg 323.00 USD
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Product Specification

Alternative Name:Tp53-induced glycolysis and apoptosis regulator
 
Host:Rabbit
 
Immunogen:Recombinant human TIGAR (aa 1-270) fused at the N-terminus to a tag.
 
UniProt ID:Q9NQ88
 
Species reactivity:Human, Mouse
 
Applications:WB
 
Recommended Dilutions/Conditions:Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Does not work for IP.
 
Purity Detail:Protein A-affinity purified.
 
Formulation:Liquid. In PBS containing 0.02% sodium azide.
 
Use/Stability:Stable for at least 1 year after receipt when stored at -20°C.
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Scientific Background:The p53 tumor-suppressor protein prevents cancer development through various mechanisms, including the induction of cell-cycle arrest, apoptosis, and the maintenance of genome stability. TIGAR (TP53-induced glycolysis and apoptosis regulator) has been identified as a p53-inducible gene. TIGAR expression decreased fructose-2,6-bisphosphate levels in cells, resulting in an inhibition of glycolysis. In addition, TIGAR expression lowered intracellular reactive oxygen species (ROS) levels and protected cells from p53-induced apoptosis. Bensaad et al., proposed that TIGAR modulates the apoptotic response to p53, thereby allowing cells to survive mild or transient stress.
 
210-947
Figure: Western Blot detection of endogenous and overexpressed TIGAR using PAb to TIGAR (AT122) (Prod. No. ALX-210-947). Method: Cell extracts from HEK 293 (lane 1), HEK 293T (lane 2) or HEK 293T transfected with a plasmid coding for human TIGAR (lane 3) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with the PAb to TIGAR (AT122) at a 1:1’000 dilution for 1 hour. Proteins were visualized using a peroxidase-conjugated PAb to rabbit IgG and a chemiluminescence detection system.
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210-947

General Literature References

TIGAR, a p53-inducible regulator of glycolysis and apoptosis: K. Bensaad, et al.; Cell 126, 107 (2006), Abstract;

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