MDA5 (human), pAb (AT113)



ALX-210-935-C100	100 µg	360.00 USD

Product Specification

Alternative Name:	Melanoma differentiation-associated gene 5, Helicard

Concentration:	1mg/ml

Purity Detail:	Epitope-affinity purified.

Formulation:	Liquid. In PBS containing 0.02% sodium azide.

Immunogen:	Recombinant human MDA5 (melanoma differentiation-associated gene 5) (aa 78-555).

Source/Host:	From rabbit.

Specificity:	Recognizes human MDA5.

Application:	Immunoprecipitation (1:100) Western Blot (1:1‘000)

Short Term Storage:	+4°C

Long Term Storage:	-20°C

Use/Stability:	Stable for at least 1 year after receipt when stored as recommended.

Handling:	Avoid freeze/thaw cycles.

Miscellaneous/General:	MDA5 is a highly conserved helicase whose ATPase activity can be stimulated by RNA and less potently by DNA. It contains two N-terminal CARD domains and a C-terminal helicase domain. The helicase domain, once freed from the CARD domains, then translocates from the cytoplasm to the nucleus where it acts on chromatin architecture. This could allow a more easy access of CAD, thereby accelerating the degradation of genomic DNA.

Product Literature References

Activation of an immunoregulatory and antiviral gene expression program in poly(I:C)-transfected human neutrophils: N. Tamassia, et al.; J. Immunol. 181, 6563 (2008), Abstract;

Distinct RIG-I and MDA5 signaling by RNA viruses in innate immunity: Y.M. Loo, et al.; J. Virol. 82, 335 (2008), Abstract;

The antiviral adaptor proteins Cardif and Trif are processed and inactivated by caspases: M. Rebsamen, et al.; Cell Death Differ. 15, 1804 (2008), Abstract;

General Literature References

Mda-5: An interferon-inducible putative RNA helicase with double-stranded RNA-dependent ATPase activity and melanoma growth-suppressive properties: D.C. Kang, et al.; PNAS 99, 637 (2002), Abstract;