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RIG-I (human) polyclonal antibody (AT111)

ALX-210-932-C100 100 µg 316.00 USD
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Product Specification

Alternative Name:RIG-1, Retinoic acid-inducible gene 1 protein, DEAD-box protein 58, DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 M, Probable ATP-dependent RNA helicase DDX58
Immunogen:Recombinant human RIG-I (aa 201-713).
UniProt ID:O95786
Species reactivity:Human
Applications:IP, WB
Recommended Dilutions/Conditions:Immunoprecipitation (1:100)
Western blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Purity Detail:Protein A-affinity purified.
Formulation:Liquid. In PBS containing 0.02% sodium azide.
Use/Stability:Stable for at least 1 year after receipt when stored at -20°C.
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Shipping:Shipped on Blue Ice
Short Term Storage:+4°C
Long Term Storage:-20°C
Scientific Background:RIG-I (retinoic acid-inducible gene I; Ddx58), a member of the DEAD-box RNA helicases, is activated by cytoplasmic dsRNA and 5’-ppp RNA produced during the viral replication. The protein is characterized by a N-terminal region with two caspase recruitment domains (CARD) and a C-terminal region harboring potential ATP-dependent RNA helicase activity. RIG-1 recruits the CARD adaptor inducing IFN-β (Cardif) in a CARD-CARD-dependent manner resulting in NF-κB and IRF3 activation.

Product Literature References

Association of a Network of Interferon-Stimulated Genes with a Locus Encoding a Negative Regulator of Non-conventional IKK Kinases and IFNB1: S. Jeidane, et al.; Cell Rep. 17, 425 (2016), Application(s): Western Immunoblot Analyses, Abstract; Full Text
ATP hydrolysis by the viral RNA sensor RIG-I prevents unintentional recognition of self-RNA: C. Lässig, et al.; eLife 4, e10859 (2015), Application(s): Dependent signaling, Abstract; Full Text
Retinoic acid-inducible gene-I-like receptor (RLR)-mediated antiviral innate immune responses in the lower respiratory tract: roles of TRAF3 and TRAF5: Y. Chiba, et al.; Biochem. Biophys. Res. Commun. 467, 191 (2015), Application(s): Immunoblot analyses of human tissue, Abstract;
Activation of an immunoregulatory and antiviral gene expression program in poly(I:C)-transfected human neutrophils: N. Tamassia, et al.; J. Immunol. 181, 6563 (2008), Abstract;

General Literature References

5’-Triphosphate RNA is the ligand for RIG-I: V. Hornung, et al.; Science 314, 994 (2006), Abstract;
RIG-I-mediated antiviral responses to single-stranded RNA bearing 5’-phosphates: A. Pichlmair, et al.; Science 314, 997 (2006), Abstract;
Virology. Sensing viral RNA amid your own: T. Fujita; Science 314, 935 (2006), Abstract;

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