Cardif (human), pAb (AT107)



ALX-210-929-C100	100 µg	380.00 USD

Product Specification

Alternative Name:	CARD adapter inducing interferon-β, IPS-1, Interferon-β promoter stimulator protein 1, MAVS, Mitochondrial antiviral signalling protein, VISA, Virus-induced signalling adapter

Concentration:	1mg/ml

Purity Detail:	Protein A-affinity purified.

Formulation:	Liquid. In PBS containing 0.02% sodium azide.

Immunogen:	Recombinant human Cardif (CARD adapter inducing interferon-β) (aa 160-450).

Source/Host:	From rabbit.

Specificity:	Recognizes human cardif. Detects a band of ~65kDa by Western blot.

Application:	Immunocytochemistry (1:500) Immunoprecipitation (1:100) Western Blot (1:2’000)

Short Term Storage:	+4°C

Long Term Storage:	-20°C

Use/Stability:	Stable for at least 1 year after receipt when stored at -20°C.

Handling:	After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Miscellaneous/General:	RIG-I (retinoic acid-inducible gene I; Ddx58) and Mda5 (melanoma differentiation-associated gene 5, also known as Ifih1 or Helicard) are proteins that sense viral replication intermediates, such as double-stranded RNA and triggers the host antiviral programs. These molecules signal the downstream activation of NF-κB and IFN regulatory factor (IRF) -3, which coordinately regulate the expression of type-I interferons. Cardif (also called VISA/IPS-1/MAVS) is a CARD (caspase activation and recruitment domain)-containing adaptor protein that interacts with the CARD domain of RIG-I and Mda5, leading to the activation of NF-κB and IRF3. Cardif is located to the mitochondrial outer membrane. Removal of the mitochondrial-targeting domain of cardif abolishes its ability to induce IFNs. Cardif is cleaved and inactivated by NS3-4A, a serine protease from hepatitis C virus known to block interferon-β production.

Western blot analysis of Cardif (human), pAb (AT107) (Prod. No. ALX-210-929): Lane 1: MW marker; Lane 2: HepG2; Lane 3: PALA; and Lane 4: HeLa. Additional bands observed probably represent isoforms or cleaved products of Cardif.

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Product Literature References

Cleavage of mitochondrial antiviral signaling protein in the liver of patients with chronic hepatitis C correlates with a reduced activation of the endogenous interferon system: P. Bellecave, et al.; Hepatology 51, 1127 (2010), Abstract;

Cleavage of the IPS-1/Cardif/MAVS/VISA does not inhibit T cell-mediated elimination of hepatitis C virus non-structural 3/4A-expressing hepatocytes: G. Ahlen, et al.; Gut 58, 560 (2009), Abstract;

Antiviral suppression vs restoration of RIG-I signaling by hepatitis C protease and polymerase inhibitors: Y. Liang, et al.; Gastroenterology 135, 1710 (2008), Abstract;

Distinct RIG-I and MDA5 signaling by RNA viruses in innate immunity: Y.M. Loo, et al.; J. Virol. 82, 335 (2008), Abstract;

The antiviral adaptor proteins Cardif and Trif are processed and inactivated by caspases: M. Rebsamen, et al.; Cell Death Differ. 15, 1804 (2008), Abstract;

Regulation of antiviral responses by a direct and specific interaction between TRAF3 and Cardif : S. K. Saha; EMBO J. 25, 3257 (2006), Abstract;

General Literature References

CARD games between virus and host get a new player: C.L. Johnson and M. Gale, Jr.; Trends Immunol. 27, 1 (2006), Abstract;

Cardif is an adaptor protein in the RIG-I antiviral pathway and is targeted by hepatitis C virus: E. Meylan, et al.; Nature 437, 1167 (2005), Abstract;

Identification and characterization of MAVS, a mitochondrial antiviral signaling protein that activates NF-kappaB and IRF 3: R.B. Seth, et al.; Cell 122, 669 (2005), Abstract;

IPS-1, an adaptor triggering RIG-I- and Mda5-mediated type I interferon induction: T. Kawai, et al.; Nat. Immunol. 6, 981 (2005), Abstract;

VISA is an adapter protein required for virus-triggered IFN-beta signaling: L.G. Xu, et al.; Mol. Cell 19, 727 (2005), Abstract;