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LRRK2 (human) polyclonal antibody (AT106)

 
ALX-210-928-C100 100 µg 363.00 USD
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Product Specification

Alternative Name:Leucine-rich repeat kinase 2, RIP7, PARK8, Dardarin
 
Host:Rabbit
 
Immunogen:Recombinant human LRRK2 (leucine-rich repeat kinase 2) (aa 1838-2133).
 
UniProt ID:Q5S007
 
Species reactivity:Human
 
Specificity:The strongest recognized epitope is PDQPRLTIPISQ (aa 1843-1854) as defined by epitope mapping.
 
Applications:IHC (PS), IP, WB
 
Recommended Dilutions/Conditions:Immunohistochemistry (paraffin sections, 1:500)
Immunoprecipitation (1:100)
Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Protein A-affinity purified.
 
Formulation:Liquid. In PBS containing 0.02% sodium azide.
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Scientific Background:LRRK2 is a large protein with multiple domains including several ankyrin, leucine-rich, and WD40 repeats, a Ras-like small GTPase family domain named Roc, and a kinase domain that is closely related to the RIP kinase domain. LRRK2 gene is expressed in brain as well as in other tissues such as lung, liver and heart. LRRK2 might be central to the pathogenesis of several major neurodegenerative diseases associated with parkinsonism. Several dominantly inherited missense mutations in the gene encoding LRRK2 have been identified in several families that exhibit a broad spectrum of neuropathological features.
 
210-928
Figure 1: Detection of human LRRK2 by PAb to LRRK2 (human) (AT106) (Prod. No. ALX-210-928). Method: Cell extracts of HEK 293T transfected with an expression vector encoding either for Flag®-tagged human LRRK2 (lane 1) or Flag®-tagged human LRRK2δN (lane 2) were run on a SDS-PAGE gel and analyzed by Western blot. After transfer, the nitrocellulose membrane was probed with PAb to LRRK2 at 1:1’000 for 1 hour at room temperature and then incubated with a goat anti-rabbit horseradish peroxidase (dilution at 1:4’000).  
210-928 ihc
Figure 2: Immunohistochemical staining of pyramidal neurons using PAb to LRRK2 (AT106) (Prod. No. ALX-210-928). Method: PAb to LRRK2 was used at a dilution of 1:500 in paraformaldehyde fixed tissue of a case of diffuse Lewy body disease.
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Product Literature References

LRRK2 in Parkinson’s disease and dementia with Lewy bodies: X. Zhu, et al.; Mol. Neurodegener. 1, 17 (2006), Abstract; Full Text

General Literature References

From The Cover: Parkinson’s disease-associated mutations in leucine-rich repeat kinase 2 augment kinase activity: A.B. West, et al.; PNAS 102, 16842 (2005), Abstract;
The RIP kinases: crucial integrators of cellular stress: E. Meylan and J. Tschopp; TIBS 30, 151 (2005), Abstract;
Cloning of the gene containing mutations that cause PARK8-linked Parkinson’s disease: C. Paisan-Ruiz, et al.; Neuron 44, 595 (2004), Abstract;

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