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Repetin polyclonal antibody (AF646)

ALX-210-925-C050 50 µg 240.00 USD
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Product Specification

Immunogen:Synthetic peptide corresponding to aa 570-584 (H570YGQTDRQGQSSHYI584) of human repetin.
UniProt ID:Q6XPR3
Species reactivity:Human, Mouse
Applications:IHC (FS), WB
Recommended Dilutions/Conditions:Immunohistochemistry (frozen sections, 1:250-1:500)
Western Blot (1:2'500-1:5,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~100kDa by Western blot.
Formulation:Liquid. In PBS containing 0.5% BSA and 0.005% sodium azide and 50% glycerol.
Use/Stability:Stable for at least 1 year after receipt when stored at -20°C.
Handling:Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Short Term Storage:+4°C
Long Term Storage:-20°C
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Figure 1: Western blot analysis of repetin expression in cytosolic (lane 1), membrane (lane 2) and cytoskeletal (lane 3) extracts from suspension-induced human keratinocytes (A) and human foreskin biopsy (B) detected with PAb to Repetin (AF646) (1:1’000). The positions of molecular weight markers in kDa are indicated on the left.
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Figure 2: Immunofluorescence analysis of repetin was carried out using PAb to Repetin (AF646) (1:100) and FITC-labeled anti-rabbit IgG antibody on frozen section of human skin (A), human foreskin (B), and human tongue (C and D).
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Product Literature References

Isolation and characterization of human repetin, a member of the fused gene family of the epidermal differentiation complex: M. Huber, et al.; J. Invest. Dermatol. 124, 998 (2005), Abstract;

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